| Revista: | Acta cirurgica brasileira |
| Base de datos: | PERIÓDICA |
| Número de sistema: | 000271812 |
| ISSN: | 0102-8650 |
| Autores: | Araujo-Filho, Irami1 Honorato-Sobrinho, Abraao Allen Rego, Amalia Cinthia Meneses do Garcia, Ana Claudia M. de Amorim Fernandes, Daniele Pimentel Cruz, Thais Medeiros Costa, Ticiana Cabral da Medeiros, Aldo Cunha |
| Instituciones: | 1Universidade Federal do Rio Grande do Norte, Natal, Rio Grande do Norte. Brasil |
| Año: | 2006 |
| Periodo: | Mar-Abr |
| Volumen: | 21 |
| Número: | 2 |
| Paginación: | 74-79 |
| País: | Brasil |
| Idioma: | Inglés |
| Tipo de documento: | Artículo |
| Enfoque: | Experimental, analítico |
| Resumen en inglés | PURPOSE: Laparoscopic surgery is associated with reduced surgical trauma, and less acute phase response, as compared with open surgery. Cytokines are important regulators of the biological response to surgical and anesthetic stress. The aim of this study was to determine if CO2 pneumoperitoneum would change cytokine expression, gas parameters and leukocyte count in septic rats. METHODS: Wistar rats were randomly assigned to five groups: control (anesthesia only), laparotomy, CO2 pneumoperitoneum, cecum ligation and puncture by laparotomy, and laparoscopic cecum ligation and puncture. After 30 min of the procedures, arterial blood samples were obtained to determine leukocytes subpopulations by hemocytometer. TNFa, IL-1b, IL-6 were determined in intraperitoneal fluid (by ELISA). Gas parameters were measured on arterial blood, intraperitoneal and subperitoneal exsudates. RESULTS: Peritoneal TNFa, IL-1b and IL-6 concentrations were lower in pneumoperitoneum rats than in all other groups (p<0.05). TNFa, IL-1b and IL-6 expression was lower in the laparoscopic than in laparotomic sepsis (p<0.05). Rats from laparoscopic cecum ligation and puncture group developed significant hypercarbic acidosis in blood and subperitoneal fluid when compared to open procedure group. Total white blood cells and lymphocytes were significantly lower in laparoscopic cecum ligation and puncture rats than in the laparotomic (p<0.01). Nevertheless, the laparotomic cecum ligation rats had a significant increase in blood neutrophils and eosinophils when compared with controls (p<0.05). CONCLUSIONS: This study demonstrates that the CO2 pneumoperitoneum reduced the inflammatory response in an animal model of peritonitis with respect to intraperitoneal cytokines, white blood cell count and clinical correlates of sepsis. The pneumoperitoneum produced hypercarbic acidosis in septic animals |
| Resumen en portugués | tambem contribuiu para instalação de acidose hipercárbica nos ratos sépticos |
| Disciplinas: | Medicina |
| Palabras clave: | Cirugía, Laparoscopia, Leucocitos, Neumoperitoneo, Bióxido de carbono, Sepsis, Acidosis |
| Keyword: | Medicine, Surgery, Leukocytes, Pneumoperitoneum, Carbon dioxide, Sepsis, Acidosis, Laparoscopy |
| Texto completo: | Texto completo (Ver HTML) |
