Caesalpinia coriaria fruits and leaves extracts possess in vitro ovicidal activity against Haemonchus contortus and Haemonchus placei



Título del documento: Caesalpinia coriaria fruits and leaves extracts possess in vitro ovicidal activity against Haemonchus contortus and Haemonchus placei
Revista: Veterinaria México OA
Base de datos: PERIÓDICA
Número de sistema: 000439052
ISSN: 2007-5472
Autores: 1
2
3
2
4
5
6
1
3
Instituciones: 1Universidad Autónoma del Estado de México, Centro Universitario UAEM Temascaltepec, Temascaltepec, Estado de México. México
2Instituto Mexicano del Seguro Social, Centro de Investigación Biomédica del Sur, Xochitepec, Morelos. México
3Instituto Nacional de Investigaciones Forestales, Agrícolas y Pecuarias, Centro Nacional de Investigación Disciplinaria en Salud Animal e Inocuidad, Jiutepec, Morelos. México
4Universidad de Matanzas "Camilo Cienfuegos", Estación Experimental de Pastos y Forrajes "Indio Hatuey", Matanzas. Cuba
5Universidad Autónoma Metropolitana, Departamento de Producción Agrícola y Animal, Ciudad de México. México
6Universidad Autónoma de San Luis Potosí, Facultad de Agronomía y Veterinaria, San Luis Potosí. México
Año:
Periodo: Oct-Dic
Volumen: 6
Número: 4
País: México
Idioma: Inglés
Tipo de documento: Artículo
Enfoque: Experimental
Resumen en inglés The ovicidal effect of hydro-alcoholic extracts from mature fruits and dried leaves of the Caesalpinia coriaria tree, against Haemonchus contortus and H. placei was evaluated by the egg hatching inhibition test (EHIT). Secondary compounds within the extracts were identified by high performance liquid chromatography. Five extract concentrations were assessed in the inhibition tests (25.0, 12.5, 6.2, 3.1 and 1.5 mg/mL), using distilled water and Thiabendazole (0.5 mg/mL) as negative and positive controls, respectively. The results were analysed under a 2 × 2 × 6 completely randomized factorial design, and mean differences were established by the Tukey’s post hoc test. The 50% (EC50) and 90% (EC90) effective concentrations for each extract were determined using a Probit analysis. A concentration-dependent ovicidal effect of hydro-alcoholic extracts from both leaves and fruits was observed, reaching a 100% efficacy against both nematode species with the 25.0 mg/mL concentration (p < 0.05). The EC50 for the fruit and leaves extracts were established as 1.63 and 3.91 mg/mL, and as 3.98 and 11.68 mg/mL, against H. contortus and H. placei, respectively. The most important secondary compounds identified in extracts were free-condensed tannins, gallic acid and methylgallate. In conclusion, hydro-alcoholic extracts from C. coriaria mature fruits and dried leaves inhibit in vitro egg hatching of H. contortus and H. placei
Disciplinas: Medicina veterinaria y zootecnia,
Biología
Palabras clave: Parasitología,
Medicina veterinaria,
Helmintos,
Caesalpinia coriaria,
Nemátodos gastrointestinales,
Haemonchus contortus,
Haemonchus placei,
Plantas medicinales,
Haemonchus contortus,
Haemonchus placei
Keyword: Parasitology,
Veterinary medicine,
Helminths,
Caesalpinia coriaria,
Gastrointestinal nematodes,
Medicinal plants
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