Use of an ELISA assay to evaluate venom, antivenom, IgG and IgM human antibody levels in serum and cerebrospinal fluid from patients bitten by Crotalus durissus terrificus in Brazil
Título del documento: Use of an ELISA assay to evaluate venom, antivenom, IgG and IgM human antibody levels in serum and cerebrospinal fluid from patients bitten by Crotalus durissus terrificus in Brazil
Revista: The journal of venomous animals and toxins
Base de datos: PERIÓDICA
Número de sistema: 000298565
ISSN: 0104-7930
Autores: 1
2
3
Instituciones: 1Universidade Estadual Paulista "Julio de Mesquita Filho", Faculdade de Medicina de Botucatu, Botucatu, Sao Paulo. Brasil
2Universidade Estadual Paulista "Julio de Mesquita Filho", Centro de Estudos de Venenos e Animais Peconhentos, Sao Paulo. Brasil
3Universidade Estadual Paulista "Julio de Mesquita Filho", Instituto de Biociencias, Botucatu, Sao Paulo. Brasil
Año:
Volumen: 2
Número: 1
Paginación: 14-27
País: Brasil
Idioma: Inglés
Tipo de documento: Artículo
Enfoque: Experimental, aplicado
Resumen en inglés A sandwich-type ELISA technique for specific and sensitive detection of Crotalus durissus terrificus venom antigens, horse-antivenom, human IgG and IgM antibodies was set up. Sixteen patients, 13 males and 3 females aged between 13 to 63 years (mean 33 ± 15) bitten by Crotalus durissus terrificus snakes were studied. Of the 15 patients, 6 had previously received anti-Crotalus venom and no seric venom was detected. For the other 9 patients studied, the venom levels ranged from 2 to 108 ng/ml according to the severity of each case. Seric antivenom was detected up to 44 days after the bite. IgM human antibody levels against Crotalus venom were higher between 3 and 18 days after specific treatment. IgG human antibody levels against Crotalus venom were detected between 30 and 90 days after envenoming. Venom and antivenom levels in cerebrospinal fluid were not observed 24 h after the bite. This suggests that neither the venom nor the antivenom is capable of crossing the blood-brain barrier. In addition, when either the venom or the antivenom is presented to the immune system cells an immune response is prepared
Disciplinas: Medicina
Palabras clave: Toxicología,
Inmunología,
Veneno de víbora,
Antivenenos,
ELISA,
Anticuerpos,
Líquido cefalorraquídeo,
Crotalus durissus terrificus
Keyword: Medicine,
Toxicology,
Immunology,
Snake venom,
Antivenoms,
ELISA,
Antibodies,
Cerebrospinal fluid,
Crotalus durissus terrificus
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