| Revista: | The journal of venomous animals and toxins |
| Base de datos: | PERIÓDICA |
| Número de sistema: | 000298615 |
| ISSN: | 0104-7930 |
| Autores: | Thomazini-Santos, I. A1 Giannini, M.J.S.M2 Toscano, E Machado, P.E.A Lima, C.R.G Barraviera, B |
| Instituciones: | 1Universidade Estadual Paulista "Julio de Mesquita Filho", Faculdade de Medicina de Botucatu, Botucatu, Sao Paulo. Brasil 2Universidade Estadual Paulista "Julio de Mesquita Filho", Faculdade de Ciencias Farmaceuticas de Araraquara, Araraquara, Sao Paulo. Brasil |
| Año: | 1998 |
| Volumen: | 1998, vol.4, p.120-136 |
| País: | Brasil |
| Idioma: | Inglés |
| Tipo de documento: | Artículo |
| Enfoque: | Experimental, aplicado |
| Resumen en inglés | The objective of this study was to evaluate the effects of the thrombin-like fraction of Crotalus durissus terrificus venom, Reptilase , and bovine thrombin of fibrinogen pools on bovine, equine, ovine, bubaline and human cryoprecipitates. The authors also made a comparative study between animal and human cryoprecipitates to see if any there was any possibility of future use in medicine. Fibrinogen levels in cryoprecipitate were studied using 48 blood samples obtained as follows:12 samples from humans, 9 from bovine, 10 from equine, 10 from ovine and 7 from bubaline. The results obtained showed average levels of 375.50 mg % for humans, 218.33 mg % for bovine, 240.80 mg % for equine, 267.70 mg % for ovine and 664.00 mg % for bubaline. Upon the formation of pools of human and animal fibrinogens, the following results were obtained: 435 mg % for humans, 444 mg % for bovine, 337 mg % for equine, 390 mg % for ovine and 530 mg % for bubaline. Statistical analysis (using the analysis of variance for entirely randomized experiment for the calculation of F statistics) demonstrated that the bubaline fibrinogen level was higher than that of human, and both were higher than those of ovine, equine, and bovine. Clotting times were determined using different dilutions of bovine thrombin, thrombin-like fraction of Crotalus durissus terrificus venom, and Reptilase . Comparing these clotting times, results for human and bovine were found to be very similar, whereas using equine, ovine, and bubaline the results above a dilution of 1:3 were markedly different. The results obtained permitted the following conclusions to be drawn show that: 1) bovine thrombin presented better interactivity with fibrinogen extracted both from human and bovine cryoprecipitates; 2) there was similar behavior when bovine thrombin was substituted for Reptilase and for the thrombin-like fraction of Crotalus durissus terrificus venom; 3) cryoprecipitate from bo |
| Disciplinas: | Medicina |
| Palabras clave: | Farmacología, Inmunología, Tiempo de coagulación, Trombina bovina, Reptilasa, Crioprecipitados, Veneno de víbora, Crotalus durissus terrificus |
| Keyword: | Medicine, Pharmacology, Immunology, Clotting time, Bovine thrombin, Reptilase, Cryoprecipitates, Snake venom, Crotalus durissus terrificus |
| Texto completo: | Texto completo (Ver HTML) |
