Rabbit antivenom efficacy against myotoxic and neurotoxic activities of Bothrops jararacussu venom and bothropstoxin-I
Título del documento: Rabbit antivenom efficacy against myotoxic and neurotoxic activities of Bothrops jararacussu venom and bothropstoxin-I
Revista: The journal of venomous animals and toxins
Base de datos: PERIÓDICA
Número de sistema: 000298759
ISSN: 0104-7930
Autores: 1



2


3
Instituciones: 1Universidade Estadual de Campinas, Departamento de Farmacologia, Campinas, Sao Paulo. Brasil
2Universidade de Sao Paulo, Faculdade de Medicina de Ribeirao Preto, Ribeirao Preto, Sao Paulo. Brasil
3Universidade Estadual de Campinas, Departamento de Histologia e Embriologia, Campinas, Sao Paulo. Brasil
Año:
Volumen: 8
Número: 2
Paginación: 226-243
País: Brasil
Idioma: Inglés
Tipo de documento: Artículo
Enfoque: Experimental, aplicado
Resumen en inglés Bothrops jararacussu venom and its major toxin bothropstoxin-I (BthTX-I) possess myotoxic and neurotoxic properties. The efficacy of a rabbit antivenom raised against B. jararacussu venom in the neutralization of physiological, biochemical, and morphological changes induced by the venom and its major toxin BthTX-I was studied in mouse isolated phrenic nerve-diaphragm (PND) and extensor digitorum longus (EDL) preparations. The times required for 50% neuromuscular blockade in PND and EDL preparations for venom were 70+11.5 (S.E.M., n=5) min and 58+8 (n=16) (50 mu g/mL), and for BthTX-I 31+6 (n=3) min and 30+3 (n=5) min (20 mu g/mL), respectively. After 120 min incubation, creatine kinase (CK) concentrations in solution containing the EDL preparations were 3464+346 U/L after exposure to venom (50 mu g/mL, n=5) and 3422+135 U/L to BthTX-I (20mu g/mL, n=4), respectively. Rabbit antivenom dose-dependently neutralized venom and toxin-induced neuromuscular blockade in both preparations and effectively prevented venom and toxin-induced CK release from EDL. Histological analysis showed that rabbit antivenom neutralized morphological damage caused by B. jararacussu venom and BthTX-I in EDL preparations. These results indicate that rabbit antivenom effectively neutralized the biological activities of B. jararacussu venom and BthTX-I
Disciplinas: Medicina
Palabras clave: Medicina experimental,
Toxicología,
Veneno de víbora,
Miotoxinas,
Neurotoxinas,
Neutralización,
Conejos,
Antivenenos,
Análisis histológico,
Bothrops jararacussu,
Viperidae
Keyword: Medicine,
Experimental medicine,
Toxicology,
Snake venom,
Myotoxins,
Neurotoxins,
Neutralization,
Rabbits,
Antivenoms,
Histological analysis,
Bothrops jararacussu,
Viperidae
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