Methodological care in the evaluation of the ld50 and of the neutralization of the lethal effect of Crotalus durissus terrificus venom by the plant Peschiera fuchsiaefolia (Apocynaceae)



Título del documento: Methodological care in the evaluation of the ld50 and of the neutralization of the lethal effect of Crotalus durissus terrificus venom by the plant Peschiera fuchsiaefolia (Apocynaceae)
Revista: The journal of venomous animals and toxins
Base de datos: PERIÓDICA
Número de sistema: 000298590
ISSN: 0104-7930
Autores: 1
2
Instituciones: 1Universidade de Sao Paulo, Faculdade de Ciencias Farmaceuticas de Ribeirao Preto, Ribeirao Preto, Sao Paulo. Brasil
2Universidade de Sao Paulo, Faculdade de Medicina de Ribeirao Preto, Ribeirao Preto, Sao Paulo. Brasil
Año:
Volumen: 3
Número: 1
Paginación: 22-31
País: Brasil
Idioma: Inglés
Tipo de documento: Artículo
Enfoque: Experimental, aplicado
Resumen en inglés In the present study, we demonstrate that the volumes in which a given protein mass of Crotalus durissus terrificus venom or of a lyophilized stabilized aqueous extract (LSAE) of Peschiera fuchsiaefolia, an antivenom agent injected intramuscularly, have a decisive influence on the results. The LD50 of C. d. terrificus venom injected i.m. in a final volume of 200µl (2µl/g) (saline solution, 0.9% NaCl) was 180µg/100g rat body weight (p<0.05, 161 to 202µg/100g body weight) and the LD50 of the venom injected i.m. in a volume of 50µl (0.5µl/g) was 120µg/100g body weight (p<0.05, 107 to 134 µg/100g body weight). The reduction of the final volume injected i.m. also required a reduced mass of LSAE necessary to neutralize the lethal effect of C. d. terrificus venom. The dose of 60mg LSAE/100g rat body weight in a final volume of 200µl administered i.m. 20 seconds after venom injection, and that of 40mg LSAE/100g body weight/200µl mixed and incubated with the venom for 1 h at 25ºC before i.m. injection were able to neutralize the lethal activity of 2LD50. However, the LSAE doses that neutralized the 2LD50 were reduced to 20mg LSAE/100g body weight in a final volume of 50µl when administered i.m. 20 seconds after venom injection and to 2.5mg LSAE/100g body weight/50µl when mixed and incubated for 1 h at 25ºC with the venom before i.m. injection. The LD50 of C. d. terrificus venom and the doses of P. fuchsiaefolia LSAE that neutralized the venom lethal activity were, therefore, significantly lower when the final volume injected i
Disciplinas: Medicina
Palabras clave: Toxicología,
Terapéutica,
Veneno de víbora,
Antídotos,
Peschiera fuchsiaefolia,
DL50,
Crotalus durissus terrificus
Keyword: Medicine,
Toxicology,
Therapeutics,
Snake venom,
Antidotes,
Peschiera fuchsiaefolia,
LD50,
Crotalus durissus terrificus
Texto completo: Texto completo (Ver HTML)