Histological aspects and protein content of Apis mellifera L. Worker venom glands: the effect of electrical shocks in summer and winter



Título del documento: Histological aspects and protein content of Apis mellifera L. Worker venom glands: the effect of electrical shocks in summer and winter
Revista: The journal of venomous animals and toxins
Base de datos: PERIÓDICA
Número de sistema: 000298696
ISSN: 0104-7930
Autores: 1
2
Instituciones: 1Universidade Federal do Acre, Departamento de Ciencias Naturais, Rio Branco, Acre. Brasil
2Universidade Estadual Paulista "Julio de Mesquita Filho", Centro de Estudos de Venenos e Animais Peconhentos, Rio Claro, Sao Paulo. Brasil
Año:
Volumen: 6
Número: 1
Paginación: 87-98
País: Brasil
Idioma: Inglés
Tipo de documento: Artículo
Enfoque: Analítico, descriptivo
Resumen en inglés This paper analyzes the summer and winter total protein content of 7, 14, 21, 28, 35, and 40-day old Apis mellifera L. worker venom glands before (control) and 24 and 96 hours after applying electrical shocks for venom extraction (experimental). During venom extraction, 7-day old workers responded more slowly and weakly to electrical shocks. This response intensifies with age, so that the workers approaching 20 days old respond faster and more aggressively to the shocks. Statistical analysis, using the non-parametric Wilcoxon and Kruskall-Wallis tests and complemented by the Jonckheere test, showed that the protein content varied from one age to another in the experimental group, which was well distinguishable from the values in the control Group in summer and winter. Summer values at all ages were always higher than those detected in winter in both groups. This variation seems to indicate the occurrence of more than one winter glandular development cycle. Histological studies showed secretion in the lumen of the control Group secretory tubes and reservoirs. The experimental group only showed vestigial secretion in the collapsed reservoirs at all ages, except at 7 days. These workers, which reacted less efficiently to electrical shocks, showed secretion in the lumen, reservoir, and tubes, even after the application of electrical shocks. During the 96 hours following the electrical shocks, a slight protein replacement was seen at some ages. This, although higher in summer than in winter, was much lower than the level detected in the
Disciplinas: Biología
Palabras clave: Anatomía e histología,
Insectos,
Abejas,
Glándulas de veneno,
Contenido proteico,
Apis mellifera,
Hymenoptera
Keyword: Biology,
Anatomy and histology,
Insects,
Bees,
Venom glands,
Protein content,
Apis mellifera,
Hymenoptera
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