| Revista: | The journal of venomous animals and toxins including tropical diseases |
| Base de datos: | PERIÓDICA |
| Número de sistema: | 000298818 |
| ISSN: | 1678-9199 |
| Autores: | Ramírez Avila, J1 Quevedo, B.E2 López, E3 Renjifo, J.M |
| Instituciones: | 1Instituto Nacional de Salud, Grupo Sueros, Bogotá. Colombia 2Universidad Nacional de Colombia, Facultad de Ingeniería, Bogotá. Colombia 3Universidad Nacional de Colombia, Facultad de Ciencias, Bogotá. Colombia |
| Año: | 2004 |
| Volumen: | 10 |
| Número: | 3 |
| Paginación: | 242-259 |
| País: | Brasil |
| Idioma: | Inglés |
| Tipo de documento: | Artículo |
| Enfoque: | Analítico, descriptivo |
| Resumen en inglés | Components with phospholipase A2 activity were isolated by gel filtration and cationic exchange chromatography from the venom of Bothrops asper snakes from Chiriguaná, Colombia (9°22´N; 73°37´W). Five fractions were obtained by the gel filtration, and PLA2 activity was found in fraction 3 (F3). In the cationic exchange chromatography, F3 showed eight components with PLA2 activity. Six of these components appeared as one band in polyacrylamide gel electrophoresis (SDS-PAGE). Fractions II and VII exhibited an optimal activity at pH 9 and 52ºC. The optimum calcium concentration for fraction II was 48 mM and for fraction VII, 384 mM. Both fractions showed thermal stability. Fraction II was stable at pH values between 2.5 and 9, and fraction VII, between 2.5 and 8. The Michaelis Menten constant (KM) was 3.5x10-3 M for fraction II and 1.6x10-3 M for fraction VII. The molecular weight was 16,000 Dalton for fraction II and 17,000 Dalton for fraction VII. Both isoenzymes did not show any toxic activity (DL50) at 5.3 and 4 µg/g. The two fractions showed different kinetic constant (KM), calcium requirement, and substrate specificity for haemolytic activity |
| Disciplinas: | Biología |
| Palabras clave: | Reptiles, Bioquímica, Veneno de víbora, Isoenzimas, Fosfolipasa A2, Bothrops asper, Viperidae |
| Keyword: | Biology, Reptiles, Biochemistry, Snake venom, Isoenzymes, Phospholipase A2, Bothrops asper, Viperidae |
| Texto completo: | Texto completo (Ver HTML) |
