| Revista: | The journal of venomous animals and toxins including tropical diseases |
| Base de datos: | PERIÓDICA |
| Número de sistema: | 000298872 |
| ISSN: | 1678-9199 |
| Autores: | Durigon, A.M1 Borja-Oliveira, C.R Belo, C.A. Dal Oshima-Franco, Y Cogo, J.C2 Lapa, A.J3 Souccar, C Rodrigues-Simioni, L |
| Instituciones: | 1Universidade Estadual de Campinas, Faculdade de Ciencias Medicas, Campinas, Sao Paulo. Brasil 2Universidade do Vale do Paraiba, Instituto de Pesquisa e Desenvolvimento, Sao Jose dos Campos, Sao Paulo. Brasil 3Universidade Federal de Sao Paulo, Escola Paulista de Medicina, Sao Paulo. Brasil |
| Año: | 2005 |
| Periodo: | Mar |
| Volumen: | 11 |
| Número: | 1 |
| Paginación: | 22-33 |
| País: | Brasil |
| Idioma: | Inglés |
| Tipo de documento: | Artículo |
| Enfoque: | Experimental, aplicado |
| Resumen en inglés | The pharmacological effects of Bothrops neuwiedi pauloensis venom on mouse phrenic nerve-diaphragm (PND) preparations were studied. Venom (20 mg/ml) irreversibly inhibited indirectly evoked twitches in PND preparations (60 ± 10% inhibition, mean ± SEM; p<0.05; n=6). At 50 mg/ml, the venom blocked indirectly and directly (curarized preparations) evoked twitches in mouse hemidiaphragms. In the absence of Ca2+, venom (50 mg/ml), produced partial blockade only after an 80 min incubation, which reached 40.3 ± 7.8% (p<0.05; n=3) after 120 min. Venom (20 mg/ml) increased (25 ± 2%, p< 0.05) the frequency of giant miniature end-plate potentials in 9 of 10 end-plates after 30 min and the number of miniature end-plate potentials which was maximum (562 ± 3%, p<0.05) after 120 min. During the same period, the resting membrane potential decreased from – 81 ± 1.4 mV to – 41.3 ± 3.6 mV 24 fibers; p<0.01; n=4) in the end-plate region and from – 77.4 ± 1.4 to –44.6 ± 3.9 mV (24 fibers; p<0.01; n=4) in regions distant from the end-plate. These results indicate that B. n. pauloensis venom acts primarily at presynaptic sites. They also suggest that enzymatic activity may be involved in this pharmacological action |
| Disciplinas: | Medicina |
| Palabras clave: | Medicina experimental, Toxicología, Veneno de víbora, Miotoxicidad, Neurotoxicidad, Inhibición presináptica, Bothrops neuwiedi pauloensis, Viperidae |
| Keyword: | Medicine, Experimental medicine, Toxicology, Snake venom, Myotoxicity, Neurotoxicity, Presynaptic inhibition, Bothrops neuwiedi pauloensis, Viperidae |
| Texto completo: | Texto completo (Ver HTML) |
