Immunization with native and cobalt 60-irradiated Crotalus durissus terrificus venom in swiss mice: assessment of the neutralizing potency of antisera
Título del documento: Immunization with native and cobalt 60-irradiated Crotalus durissus terrificus venom in swiss mice: assessment of the neutralizing potency of antisera
Revista: The journal of venomous animals and toxins including tropical diseases
Base de datos: PERIÓDICA
Número de sistema: 000298859
ISSN: 1678-9199
Autores: 1
2
3
Instituciones: 1Universidade Estadual Paulista "Julio de Mesquita Filho", Faculdade de Medicina de Botucatu, Botucatu, Sao Paulo. Brasil
2Comissao Nacional de Energia Nuclear, Instituto de Pesquisas Energeticas e Nucleares, Sao Paulo. Brasil
3Universidade Estadual Paulista "Julio de Mesquita Filho", Faculdade de Engenharia, Bauru, Sao Paulo. Brasil
Año:
Periodo: Sep
Volumen: 11
Número: 3
Paginación: 299-314
País: Brasil
Idioma: Inglés
Tipo de documento: Artículo
Enfoque: Experimental
Resumen en inglés ELISA was used to evaluate, follow, and compare the humoral immune response of Swiss mice during hyperimmunization with natural and Cobalt 60-irradiated (60Co) Crotalus durissus terrificus venom. Potency and neutralization were evaluated by in vitro challenges. After hyperimmunization, immunity was observed by "in vivo" challenge and the side effects were assessed. The animals immunization with one LD50 of the venom was on days one, 15, 21, 30, and 45, when blood samples were collected; the challenges occurred on the 60th day. Results showed that ELISA was efficient in evaluating, following, and comparing mouse immune response during hyperimmunization. Serum titers produced with natural venom were similar to those produced with irradiated venom. Immunogenic capacity was maintained after 60Co irradiation. Serum produced from Crotalus durissus terrificus irradiated venom showed higher potency and neutralization capacity than that from natural venom. All antibodies were able to neutralize five LD50 from these venoms. Clinical alterations were minimum during hyperimmunization with irradiated venom
Disciplinas: Medicina
Palabras clave: Toxicología,
Inmunología,
Veneno de víbora,
Hiperinmunización,
ELISA,
Neutralización sérica,
Destoxificación,
Crotalus durissus terrificus,
Crotalidae
Keyword: Medicine,
Toxicology,
Immunology,
Snake venom,
Hyperimmunization,
ELISA,
Serum neutralization,
Detoxification,
Crotalus durissus terrificus,
Crotalidae
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