| Revista: | The journal of venomous animals and toxins including tropical diseases |
| Base de datos: | PERIÓDICA |
| Número de sistema: | 000298850 |
| ISSN: | 1678-9199 |
| Autores: | More, S.S1 Mirajkar, K.K Gadag, J.R Menon, K.S2 Mathew, M.K |
| Instituciones: | 1Karnataka University, Toxinology Division, Dharwad, Karnataka. India 2Tata Institute of Fundamental Research, National Centre for Biological Sciences, Bangalore, Karnataka. India |
| Año: | 2005 |
| Periodo: | Sep |
| Volumen: | 11 |
| Número: | 3 |
| Paginación: | 315-335 |
| País: | Brasil |
| Idioma: | Inglés |
| Tipo de documento: | Artículo |
| Enfoque: | Experimental, aplicado |
| Resumen en inglés | A peptide toxin was isolated from the venom of Palamneus gravimanus, the Indian black scorpion, to block human Kv1.1 channels expressed in Xenopus laevis oocytes. A 4.5 kD peptide (toxin), as confirmed by SDS-PAGE, was purified to homogeneity by ion exchange chromatography using CM-Sephadex C-25 followed by Sephadex G-50 gel filtration. Palamneus gravimanus toxin (PGT) selectively blocks the human cloned voltage-gated potassium channel hKv1.1 in a two-electrode voltage-clamp (TEVC) technique. The results obtained indicate that the toxin blocks the hKv1.1 channel at a nanomolar concentration range (Ki value of 10 nM) of the peptide to the external side of the cell. The blockage seems to be voltage-dependent. Comparative structure of PGT (a 4.5 kD peptide) with BTK-2 suggests a close relationship; therefore this toxin can be employed to investigate the hKv1.1 channel structure |
| Disciplinas: | Biología |
| Palabras clave: | Quelicerados, Bioquímica, Veneno de escorpión, Toxinas, Canales de potasio, Bloqueo, Palamneus gravimanus, Scorpionida |
| Keyword: | Biology, Chelicerata, Biochemistry, Scorpion venom, Toxins, Potassium channels, Blockade, Palamneus gravimanus, Scorpionida |
| Texto completo: | Texto completo (Ver HTML) |
