| Revista: | Scientia Agricola |
| Base de datos: | PERIÓDICA |
| Número de sistema: | 000455502 |
| ISSN: | 0103-9016 |
| Autores: | Almeida, Dênia Pires de1 Castro, Isabel Samila Lima1 Mendes, Tiago Antônio de Oliveira2 Alves, Danúbia Rodrigues1 Barka, Geleta Dugassa1 Barreiros, Pedro Ricardo Rossi Marques1 Zambolim, Laércio3 Sakiyama, Ney Sussumu4 Caixeta, Eveline Teixeira5 |
| Instituciones: | 1Universidade Federal de Vicosa, Instituto de Biotecnologia Aplicada a Agropecuaria, Vicosa, Minas Gerais. Brasil 2Universidade Federal de Vicosa, Departamento de Bioquimica e Biologia Molecular, Vicosa, Minas Gerais. Brasil 3Universidade Federal de Vicosa, Departamento de Fitopatologia, Vicosa, Minas Gerais. Brasil 4Universidade Federal de Vicosa, Departamento de Fitotecnia, Vicosa, Minas Gerais. Brasil 5Empresa Brasileira de Pesquisa Agropecuaria, Cafe, Brasilia, Distrito Federal. Brasil |
| Año: | 2021 |
| Volumen: | 78 |
| Número: | 6 |
| País: | Brasil |
| Idioma: | Inglés |
| Tipo de documento: | Artículo |
| Enfoque: | Experimental, analítico |
| Resumen en inglés | The biotrophic fungus Hemileia vastatrix causes coffee leaf rust (CLR), one of the most devastating diseases in Coffea arabica . Coffee, like other plants, has developed effective mechanisms to recognize and respond to infections caused by pathogens. Plant resistance gene analogs (RGAs) have been identified in certain plants as candidates for resistance ( R ) genes or membrane receptors that activate the R genes. The RGAs identified in different plants possess conserved domains that play specific roles in the fight against pathogens. Despite the importance of RGAs, in coffee plants these genes and other molecular mechanisms of disease resistance are still unknown. This study aimed to sequence and characterize candidate genes from coffee plants with the potential for involvement in resistance to H. vastatrix . Sequencing was performed based on a library of bacterial artificial chromosomes (BAC) of the coffee clone ‘Híbrido de Timor’ (HdT) CIFC 832/2 and screened using a functional marker. Two RGAs, HdT_LRR_RLK1 and HdT_LRR_RLK2, containing the motif of leucine-rich repeat-like kinase (LRR-RLK) were identified. Based on the presence or absence of the HdT_LRR_RLK2 RGA in a number of differential coffee clones containing different combinations of the rust resistance gene, these RGAs did not correspond to any resistance gene already characterized (SH1-9). These genes were also analyzed using qPCR and demonstrated a major expression peak at 24 h after inoculation in both the compatible and incompatible interactions between coffee and H. vastatrix . These results are valuable information for breeding programs aimed at developing CLR-resistant cultivars, in addition to enabling a better understanding of the interactions between coffee and H. vastatrix |
| Disciplinas: | Agrociencias, Biología |
| Palabras clave: | Hongos, Genética, Plantas para uso industrial, Café, Coffea arabica, Resistencia a enfermedades, Marcadores moleculares, Mejoramiento genético, Hongos fitopatógenos |
| Keyword: | Fungi, Genetics, Plants for industrial use, Coffee, Coffea arabica, Disease resistance, Molecular markers, Phytopathogenic fungi, Plant breeding |
| Texto completo: | Texto completo (Ver HTML) Texto completo (Ver PDF) |
