| Revista: | Revista brasileira de hematologia e hemoterapia |
| Base de datos: | PERIÓDICA |
| Número de sistema: | 000306046 |
| ISSN: | 1516-8484 |
| Autores: | Campos, Lizia M.F.R1 Dias, Francisca L Mendes, Marcel2 |
| Instituciones: | 1Universidade Federal do Triangulo Mineiro, Uberaba, Minas Gerais. Brasil 2Universidade de Uberaba, Faculdade de Biomedicina, Uberaba, Minas Gerais. Brasil |
| Año: | 2005 |
| Periodo: | Jul-Sep |
| Volumen: | 27 |
| Número: | 3 |
| Paginación: | 210-212 |
| País: | Brasil |
| Idioma: | Portugués |
| Tipo de documento: | Correspondencia |
| Enfoque: | Descriptivo |
| Resumen en inglés | Glucose 6-Phosphate desidrogenase (G-6-PD) is a cytoplasmic enzyme present in all cells whose main function, in erythrocytes, is to protect against oxygen free radicals, through the production of NADPH. G-6-PD deficiency is the most common enzymopathy in human beings, affecting about 2 to 3% of the population worldwide. The necessity of neonatal screening of G-6-PD has been discussed in Brazil because of this high frequency. We used the Intercientífica test, which utilizes G-6-PD and in the presence of NAD, catalyzes the oxidation of G-6-P to 6-fosfogluconato. The NADPH produced is kinetically measured at 340 nm, correcting the enzymatic activity according to the concentration of the Hb at 405 nm. As controls, lyophilized erythrocytes were used. The samples were collected from the umbilical cord in EDTA. Of the 506 samples that were analyzed, 44 presented activity of the G-6-PD of more than 17.1 U/gHb (91.5%) were compatible with normal enzymatic activity; 33 (females) and 01 (male) with activity between 5.7 and 17.1 U/gHb (6.5%) were compatible with partial deficiency and 10 (male) with less than 5.7 U/gHb enzymatic activity (1.9%), compatible with total deficiency. With these results we concluded that partial and total deficiency of G-6-PD in the city of Uberaba is 8.4% |
| Disciplinas: | Medicina |
| Palabras clave: | Hematología, Salud pública, Glucosa 6-fosfato deshidrogenasa, Deficiencia, Tamizaje neonatal |
| Keyword: | Medicine, Hematology, Public health, Glucose-6-phosphate dehydrogenase, Deficiency, Neonatal screening |
| Texto completo: | Texto completo (Ver HTML) |
