| Revista: | Pesquisa veterinaria brasileira |
| Base de datos: | PERIÓDICA |
| Número de sistema: | 000301621 |
| ISSN: | 0100-736X |
| Autores: | Spilki, Fernando R1 Spilki, Fernando R2 Silva, Alessandra D Batista, Helena Beatriz C. Ruthner Oliveira, Anna P Winkelmann, Evandro3 Franco, Ana C Porciuncula, Jorge A4 Roehe, Paulo Michel |
| Instituciones: | 1Universidade Estadual de Campinas, Laboratorio de Virologia Animal, Campinas, Sao Paulo. Brasil 2Instituto de Pesquisas Veterinarias "Desiderio Finamor", Porto Alegre, Rio Grande do Sul. Brasil 3Universidade Federal de Santa Maria, Centro de Ciencias Rurais, Santa Maria, Rio Grande do Sul. Brasil 4Fundacao Estadual de Pesquisa Agropecuaria, Estacao Experimental de Sao Gabriel, Sao Gabriel, Rio Grande do Sul. Brasil |
| Año: | 2005 |
| Periodo: | Ene-Mar |
| Volumen: | 25 |
| Número: | 1 |
| Paginación: | 54-58 |
| País: | Brasil |
| Idioma: | Inglés |
| Tipo de documento: | Artículo |
| Enfoque: | Experimental |
| Resumen en inglés | Bovine herpesvirus type 1 (BoHV-1) is recognized as a major cause of respiratory, reproductive disease and abortion in cattle. Vaccination is widely applied to minimize losses induced by BoHV-1 infections; however, vaccination of dams during pregnancy with modified live virus (MLV) vaccines has been occasionally associated to abortions. We have previously reported the development of a BoHV-1 recombinant virus, constructed with basis on a Brazilian BoHV-1 (Franco et al. 2002a) from which the gene coding for glycoprotein E (gE) was deleted (gE-) by genetic manipulation. Such recombinant has been previously evaluated in its potential as a differential vaccine (gE- vaccine) that allows differentiation between vaccinated and infected animals. Here, in the first part of the present study, the safety of the gE- vaccine during pregnancy was evaluated by the intramuscular inoculation of 10(7.4) tissue culture 50 % infective doses (TCID50) of the virus into 22 pregnant dams (14 BoHV-1 seronegative; 8 seropositive), at different stages of gestation. Other 15 pregnant dams were kept as non-vaccinated controls. No abortions, stillbirths or fetal abnormalities were seen after vaccination. Seroconversion was observed in both groups of previously seronegative vaccinated animals. In the second part of the study, the potential of the gE- vaccine virus to spread among beef cattle under field conditions was examined. Four heifers were inoculated intranasally with a larger amount (10(7,6) TCID50) of the gE- vaccine (to increase chances of transmission) |
| Disciplinas: | Medicina veterinaria y zootecnia, Biología |
| Palabras clave: | Bovinos, Virus, Vacas, Herpesvirus bovino tipo 1 (BHV-1), Vacunas recombinantes, Preñez |
| Keyword: | Veterinary medicine and animal husbandry, Biology, Bovines, Virus, Cows, Bovine herpesvirus type 1 (BHV-1), Recombinant vaccines, Pregnancy |
| Texto completo: | Texto completo (Ver HTML) |
