| Revista: | Memorias do Instituto Oswaldo Cruz |
| Base de datos: | PERIÓDICA |
| Número de sistema: | 000446394 |
| ISSN: | 0074-0276 |
| Autores: | Panzera, Yanina1 Ramos, Natalia1 Calleros, Lucía1 Marandino, Ana1 Tomás, Gonzalo1 Techera, Claudia1 Grecco, Sofía1 Frabasile, Sandra1 Fuques, Eddie1 Coppola, Leticia2 Goñi, Natalia2 Ramas, Viviana2 Sorhouet, Cecilia2 Bormida, Victoria2 Burgueño, Analía2 Brasesco, María2 Garland, Maria Rosa2 Molinari, Sylvia2 Perez, Maria Teresa2 Somma, Rosina2 Somma, Silvana2 Morel, Maria Noelia2 Mogdasy, Cristina2 Chiparelli, Héctor2 Arbiza, Juan1 Delfraro, Adriana1 Pérez, Ruben1 |
| Instituciones: | 1Universidad de la República, Facultad de Ciencias, Montevideo. Uruguay 2Ministerio de Salud Pública, Centro Nacional de Referencia de Influenza y Otros Virus Respiratorios, Montevideo. Uruguay |
| Año: | 2021 |
| Volumen: | 116 |
| País: | Brasil |
| Idioma: | Inglés |
| Tipo de documento: | Artículo |
| Enfoque: | Experimental, aplicado |
| Resumen en inglés | BACKGROUND Evolutionary changes in severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) include indels in non-structural, structural, and accessory open reading frames (ORFs) or genes. OBJECTIVES We track indels in accessory ORFs to infer evolutionary gene patterns and epidemiological links between outbreaks. METHODS Genomes from Coronavirus disease 2019 (COVID-19) case-patients were Illumina sequenced using ARTIC_V3. The assembled genomes were analysed to detect substitutions and indels. FINDINGS We reported the emergence and spread of a unique 4-nucleotide deletion in the accessory ORF6, an interesting gene with immune modulation activity. The deletion in ORF6 removes one repeat unit of a two 4-nucleotide repeat, which shows that directly repeated sequences in the SARS-CoV-2 genome are associated with indels, even outside the context of extended repeat regions. The 4-nucleotide deletion produces a frameshifting change that results in a protein with two inserted amino acids, increasing the coding information of this accessory ORF. Epidemiological and genomic data indicate that the deletion variant has a single common ancestor and was initially detected in a health care outbreak and later in other COVID-19 cases, establishing a transmission cluster in the Uruguayan population. MAIN CONCLUSIONS Our findings provide evidence for the origin and spread of deletion variants and emphasise indels’ importance in epidemiological studies, including differentiating consecutive outbreaks occurring in the same health facility |
| Disciplinas: | Medicina |
| Palabras clave: | Virus, Genética, COVID-19, Mutaciones génicas, Indeles, Genes accesorios, Secuencia repetitiva |
| Keyword: | Virus, Genetics, COVID-19, Gene mutations, Indels, Accessory genes, Repetitive sequence |
| Texto completo: | Texto completo (Ver HTML) Texto completo (Ver PDF) |
