| Revista: | Memorias do Instituto Oswaldo Cruz |
| Base de datos: | PERIÓDICA |
| Número de sistema: | 000446477 |
| ISSN: | 0074-0276 |
| Autores: | Coatsworth, Heather1 Caicedo, Paola A2 Winsor, Geoffrey3 Brinkman, Fiona3 Ocampo, Clara B2 Lowenberger, Carl1 |
| Instituciones: | 1Simon Fraser University, Department of Biological Sciences, Burnaby, British Columbia. Canadá 2Universidad ICESI, Facultad de Ciencias Naturales, Cali, Valle del Cauca. Colombia 3Simon Fraser University, Department of Molecular Biology and Biochemistry, Burnaby, British Columbia. Canadá |
| Año: | 2021 |
| Volumen: | 116 |
| País: | Brasil |
| Idioma: | Inglés |
| Tipo de documento: | Artículo |
| Enfoque: | Experimental, aplicado |
| Resumen en inglés | BACKGROUND Forty percent of the world’s population live in areas where they are at risk from dengue fever, dengue hemorrhagic fever, and dengue shock syndrome. Dengue viruses are transmitted primarily by the mosquito Aedes aegypti. In Cali, Colombia, approximately 30% of field collected Ae. aegypti are naturally refractory to all four dengue serotypes. OBJECTIVES Use RNA-sequencing to identify those genes that determine refractoriness in feral mosquitoes to dengue. This information can be used in gene editing strategies to reduce dengue transmission. METHODS We employed a full factorial design, analyzing differential gene expression across time (24, 36 and 48 h post bloodmeal), feeding treatment (blood or blood + dengue-2) and strain (susceptible or refractory). Sequences were aligned to the reference Ae. aegypti genome for identification, assembled to visualize transcript structure, and analyzed for dynamic gene expression changes. A variety of clustering techniques was used to identify the differentially expressed genes. FINDINGS We identified a subset of genes that likely assist dengue entry and replication in susceptible mosquitoes and contribute to vector competence. MAIN CONCLUSIONS The differential expression of specific genes by refractory and susceptible mosquitoes could determine the phenotype, and may be used to in gene editing strategies to reduce dengue transmission |
| Disciplinas: | Biología, Medicina |
| Palabras clave: | Microbiología, Genética, Insectos, Dengue, Mosquitos, Secuenciamiento génico, ARN, Inmunidad innata, Aedes aegypti, Diptera |
| Keyword: | Microbiology, Insects, Genetics, Dengue, Mosquitoes, Gene sequencing, RNA, Innate immunity, Aedes aegypti, Diptera |
| Texto completo: | Texto completo (Ver HTML) Texto completo (Ver PDF) |
