Molecular evidence of high-risk human papillomavirus infection in colorectal tumours from Cuban patients
Título del documento: Molecular evidence of high-risk human papillomavirus infection in colorectal tumours from Cuban patients
Revista: Memorias do Instituto Oswaldo Cruz
Base de datos: PERIÓDICA
Número de sistema: 000401451
ISSN: 0074-0276
Autores: 1
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2
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Instituciones: 1Instituto de Medicina Tropical "Pedro Kouri", Departamento de Virología, La Habana. Cuba
2Instituto de Gastroenterología, La Habana. Cuba
Año:
Periodo: Dic
Volumen: 111
Número: 12
Paginación: 731-736
País: Brasil
Idioma: Inglés
Tipo de documento: Artículo
Enfoque: Experimental, aplicado
Resumen en inglés The association between colorectal cancer and human papillomavirus (HPV) infection is still unproven. The aim of this study was to investigate the presence of high-risk HPV (HR-HPV) DNA in colorectal tissues from Cuban patients. A total of 63 colorectal formalin-fixed paraffin-embedded tissues were studied (24 adenocarcinoma, 18 adenoma, and 21 colorectal tissues classified as benign colitis). DNA from colorectal samples was analysed by quantitative real-time polymerase chain reaction to detect the most clinically relevant high HR-HPV types (HPV-16, -18, -31, -33, -45, -52, and -58). Associations between histologic findings and other risk factors were also analysed. Overall, HPV DNA was detected in 23.8% (15/63) of the samples studied. Viral infections were detected in 41.7% of adenocarcinoma (10/24) and 27.7% of adenoma cases (5/18). HPV DNA was not found in any of the negative cases. An association between histological diagnosis of adenocarcinoma and HPV infection was observed (odd ratio = 4.85, 95% confidence interval = 1.40-16.80, p = 0.009). The only genotypes identified were HPV 16 and 33. Viral loads were higher in adenocarcinoma, and these cases were associated with HPV 16. This study provides molecular evidence of HR-HPV infection in colorectal adenocarcinoma tissues from Cuban patients
Disciplinas: Medicina
Palabras clave: Gastroenterología,
Oncología,
Virus del papiloma humano,
Cáncer colorrectal,
Factores de riesgo,
Reacción en cadena de la polimerasa (PCR)
Keyword: Medicine,
Gastroenterology,
Oncology,
Human papillomavirus,
Colorectal cancer,
Risk factors,
Polymerase chain reaction (PCR)
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