| Revista: | Memorias do Instituto Oswaldo Cruz |
| Base de datos: | PERIÓDICA |
| Número de sistema: | 000352155 |
| ISSN: | 0074-0276 |
| Autores: | Tavares, Talissa de Moraes1 Brito, Wilia Marta Elsner Diederichsen de1 Fiaccadori, Fabíola Souza1 Freitas, Erika Regina Leal de1 Parente, Juliana Alves2 Costa, Paulo Sergio Sucasas da3 Giugliano, Loreny Gimenes4 Andreasi, Marcia Sueli Assis5 Soares, Celia Maria de Almeida2 Cardoso, Divina das Dores de Paula1 |
| Instituciones: | 1Universidade Federal de Goias, Laboratorio de Virologia, Goiania, Goias. Brasil 2Universidade Federal de Goias, Laboratorio de Biologia Molecular, Goiania, Goias. Brasil 3Universidade Federal de Goias, Faculdade de Medicina, Goiania, Goias. Brasil 4Universidade de Brasilia, Departamento de Biologia Celular, Brasilia, Distrito Federal. Brasil 5Universidade Federal de Mato Grosso do Sul, Departamento de Patologia, Campo Grande, Mato Grosso do Sul. Brasil |
| Año: | 2008 |
| Periodo: | May |
| Volumen: | 103 |
| Número: | 3 |
| Paginación: | 288-294 |
| País: | Brasil |
| Idioma: | Inglés |
| Tipo de documento: | Artículo |
| Enfoque: | Experimental |
| Resumen en inglés | Nonstructural protein 4 (NSP4), encoded by group A rotavirus genome segment 10, is a multifunctional protein and the first recognized virus-encoded enterotoxin. The NSP4 gene has been sequenced, and five distinct genetic groups have been described: genotypes A-E. NSP4 genotypes A, B, and C have been detected in humans. In this study, the NSP4-encoding gene of human rotavirus strains of different G and P genotypes collected from children between 1987 and 2003 in three cities of West Central region of Brazil was characterized. NSP4 gene of 153 rotavirus-positive fecal samples was amplified by reverse transcriptase-polymerase chain reaction and then sequenced. For phylogenetic analysis, NSP4 nucleotide sequences of these samples were compared to nucleotide sequences of reference strains available in GenBank. Two distinct NSP4 genotypes could be identified: 141 (92.2%) sequences clustered with NSP4 genotype B, and 12 sequences (7.8%) clustered with NSP4 genotype A. These results reinforce that further investigations are needed to assess the validity of NSP4 as a suitable target for epidemiologic surveillance of rotavirus infections and vaccine development |
| Disciplinas: | Biología, Química |
| Palabras clave: | Genética, Virus, Bioquímica, Grupo rotavirus A, Gen NSP4, Genotipos, Región Centro-Oeste, Brasil |
| Keyword: | Biology, Chemistry, Genetics, Virus, Biochemistry, Group A rotavirus, NSP4 gene, Genotypes, West Central region, Brazil |
| Texto completo: | Texto completo (Ver PDF) |
