| Revista: | Memorias do Instituto Oswaldo Cruz |
| Base de datos: | PERIÓDICA |
| Número de sistema: | 000420342 |
| ISSN: | 0074-0276 |
| Autores: | Guimarães, Agna Cristina1 Nogueira, Paula Monalisa2 Silva, Soraia de Oliveira1 Sadlova, Jovana3 Pruzinova, Katerina3 Hlavacova, Jana3 Melo, Maria Norma1 Soares, Rodrigo Pedro2 |
| Instituciones: | 1Universidade Federal de Minas Gerais, Departamento de Parasitologia, Belo Horizonte, Minas Gerais. Brasil 2Fundacao Oswaldo Cruz, Instituto Rene Rachou, Belo Horizonte, Minas Gerais. Brasil 3Charles University, Faculty of Science, Praga. República Checa |
| Año: | 2018 |
| Volumen: | 113 |
| Número: | 5 |
| País: | Brasil |
| Idioma: | Inglés |
| Tipo de documento: | Artículo |
| Enfoque: | Experimental, aplicado |
| Resumen en inglés | Leishmania major is an Old World species causing cutaneous leishmaniasis and is transmitted by Phlebotomus papatasi and Phlebotomus duboscqi. In Brazil, two isolates from patients who never left the country were characterised as L. major-like (BH49 and BH121). Using molecular techniques, these isolates were indistinguishable from the L. major reference strain (FV1). OBJECTIVES We evaluated the lipophosphoglycans (LPGs) of the strains and their behaviour in Old and New World sand fly vectors. METHODS LPGs were purified, and repeat units were qualitatively evaluated by immunoblotting. Experimental in vivo infection with L. major-like strains was performed in Lutzomyia longipalpis (New World, permissive vector) and Ph. papatasi (Old World, restrictive or specific vector). FINDINGS The LPGs of both strains were devoid of arabinosylated side chains, whereas the LPG of strain BH49 was more galactosylated than that of strain BH121. All strains with different levels of galactosylation in their LPGs were able to infect both vectors, exhibiting colonisation of the stomodeal valve and metacyclogenesis. The BH121 strain (less galactosylated) exhibited lower infection intensity compared to BH49 and FV1 in both vectors. MAIN CONCLUSIONS Intraspecific variation in the LPG of L. major-like strains occur, and the different galactosylation levels affected interactions with the invertebrate host |
| Disciplinas: | Medicina |
| Palabras clave: | Parasitología, Lipofosfoglicano, Galactosilación, Huésped-parásito, Leishmania major, Phlebotomus papatasi, Lutzomyia longipalpis |
| Keyword: | Parasitology, Lipophosphoglycan, Galactosylation, Host-parasite, Leishmania major, Phlebotomus papatasi, Lutzomyia longipalpis |
| Texto completo: | Texto completo (Ver HTML) |
