| Revista: | Memorias do Instituto Oswaldo Cruz |
| Base de datos: | PERIÓDICA |
| Número de sistema: | 000446478 |
| ISSN: | 0074-0276 |
| Autores: | Sáez Alquezar, Amadeo1 Junqueira, Angela Cristina Verissimo2 Durans, Andressa da Matta3 Guimarães, André Valpassos1 Corrêa, José Abol1 Borges Pereira, José2 Zauza, Patrícia Lago2 Cabello, Pedro Hernan2 Albajar Viñas, Pedro5 Provance-Junior, David William3 Coura, José Rodrigues2 |
| Instituciones: | 1Sociedade Brasileira de Analises Clinicas, Programa Nacional de Controle de Qualidade, Rio de Janeiro. Brasil 2Fundacao Oswaldo Cruz, Instituto Oswaldo Cruz, Rio de Janeiro. Brasil 3Fundacao Oswaldo Cruz, Centro de Desenvolvimento Tecnologico em Saude, Rio de Janeiro. Brasil 4Universidade do Grande Rio, Laboratorio de Genetica, Duque de Caxias, Rio de Janeiro. Brasil |
| Año: | 2021 |
| Volumen: | 116 |
| País: | Brasil |
| Idioma: | Inglés |
| Tipo de documento: | Artículo |
| Enfoque: | Aplicado, descriptivo |
| Resumen en inglés | BACKGROUND Chagas disease, caused by Trypanosoma cruzi, affects nearly six million people worldwide. Various serological tests have been developed for its diagnosis. OBJECTIVE Examine the performance of a set of commercial immunological assays in relation to the geographical origin of the patient sample comparing four states of Brazil: Amazonas (AM), Mato Grosso do Sul (MS), Minas Gerais (MG) and Piauí (PI). METHODS Seven immunoassays were employed to detect anti-T. cruzi IgG antibodies in 379 patient samples that had been previously diagnosed using the two-step protocol required by the Brazilian Ministry of Health. FINDINGS A significant variation in the percent reactive was calculated for the samples from AM and MS, while the PI and MG showed a significant variation in the percent non-reactive. The average reactivity index was significantly higher for samples from the states of PI and MG states than AM and MS. MAIN CONCLUSIONS All tests presented a satisfactory performance overall. Yet, variations were observed that were associated to the region of origin of the samples. Our analyses suggest that future evaluations of immunoassays should include a sampling of sera from regions where the test will be applied in addition to the available International Biological Reference Standards |
| Disciplinas: | Medicina |
| Palabras clave: | Microbiología, Diagnóstico, Enfermedad de Chagas, Diagnóstico serológico, Pruebas diagnósticas, Inmunoensayo, Trypanosoma cruzi |
| Keyword: | Microbiology, Diagnosis, Chagas disease, Serological diagnosis, Diagnostic tests, Immunoassay, Trypanosoma cruzi |
| Texto completo: | Texto completo (Ver HTML) Texto completo (Ver PDF) |
