DNA multigene sequencing of topotypic specimens of the fascioliasis vector Lymnaea diaphana and phylogenetic analysis of the genus Pectinidens (Gastropoda)
Título del documento: DNA multigene sequencing of topotypic specimens of the fascioliasis vector Lymnaea diaphana and phylogenetic analysis of the genus Pectinidens (Gastropoda)
Revista: Memorias do Instituto Oswaldo Cruz
Base de datos: PERIÓDICA
Número de sistema: 000351343
ISSN: 0074-0276
Autores: 1
2
1
1
Instituciones: 1Universidad de Valencia, Facultad de Farmacia, Valencia. España
2Universidad Juan Agustín Maza, Facultad de Ciencias Veterinarias, Guaymallén, Mendoza. Argentina
Año:
Periodo: Feb
Volumen: 107
Número: 1
País: Brasil
Idioma: Inglés
Tipo de documento: Artículo
Enfoque: Experimental, analítico
Resumen en inglés Freshwater lymnaeid snails are crucial in defining transmission and epidemiology of fascioliasis. In South America, human endemic areas are related to high altitudes in Andean regions. The species Lymnaea diaphana has, however, been involved in low altitude areas of Chile, Argentina and Peru where human infection also occurs. Complete nuclear ribosomal DNA 18S, internal transcribed spacer (ITS)-2 and ITS-1 and fragments of mitochondrial DNA 16S and cytochrome c oxidase (cox)1 genes of L. diaphana specimens from its type locality offered 1,848, 495, 520, 424 and 672 bp long sequences. Comparisons with New and Old World Galba/Fossaria, Palaearctic stagnicolines, Nearctic stagnicolines, Old World Radix and Pseudosuccinea allowed to conclude that (i) L. diaphana shows sequences very different from all other lymnaeids, (ii) each marker allows its differentiation, except cox1 amino acid sequence, and (iii) L. diaphana is not a fossarine lymnaeid, but rather an archaic relict form derived from the oldest North American stagnicoline ancestors. Phylogeny and large genetic distances support the genus Pectinidens as the first stagnicoline representative in the southern hemisphere, including colonization of extreme world regions, as most southern Patagonia, long time ago. The phylogenetic link of L. diaphana with the stagnicoline group may give light to the aforementioned peculiar low altitude epidemiological scenario of fascioliasis
Disciplinas: Biología,
Medicina
Palabras clave: Moluscos,
Parasitología,
Salud pública,
Lymnaea diaphana,
Pectinidens,
Lymnaeidae,
Filogenia,
ADN,
Vectores biológicos,
Fascioliasis
Keyword: Biology,
Medicine,
Molluscs,
Parasitology,
Public health,
Lymnaea diaphana,
Pectinidens,
Lymnaeidae,
Phylogeny,
DNA,
Biological vectors,
Fascioliasis
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