| Revista: | Memorias do Instituto Oswaldo Cruz |
| Base de datos: | PERIÓDICA |
| Número de sistema: | 000437066 |
| ISSN: | 0074-0276 |
| Autores: | Iani, Felipe Campos de Melo1 Caetano, Ana Carolina Barbosa2 Cocovich, Jéssica Caroline Wenceslau2 Amancio, Frederico Figueiredo3 Pereira, Maira Alves1 Adelino, Talita Emile Ribeiro1 Caldas, Sérgio4 Silva, Marcos Vinícius Ferreira1 Pereira, Glauco de Carvalho1 Duarte, Myrian Morato1 |
| Instituciones: | 1Fundacao Ezequiel Dias, Instituto Octavio Magalhaes, Belo Horizonte, Minas Gerais. Brasil 2Centro Universitario de Belo Horizonte, Belo Horizonte, Minas Gerais. Brasil 3Universidade Federal de Minas Gerais, Faculdade de Medicina, Belo Horizonte, Minas Gerais. Brasil 4Fundacao Ezequiel Dias, Diretoria de Pesquisa e Desenvolvimento, Belo Horizonte, Minas Gerais. Brasil |
| Año: | 2020 |
| Volumen: | 115 |
| País: | Brasil |
| Idioma: | Inglés |
| Tipo de documento: | Artículo |
| Enfoque: | Analítico, descriptivo |
| Resumen en inglés | The heat-labile nature of Dengue virus (DENV) in serum samples must be considered when applying routine diagnostic tests to avoid issues that could impact the accuracy of test results with direct implications for case management and disease reporting. OBJECTIVES To check if pre-analytical variables, such as storage time and temperature, have an impact on the accuracy of the main routine diagnostic tests for dengue. METHODS Virus isolation, reverse transcription real-time polymerase chain reaction (RT-PCR) and NS1 enzyme-linked immunosorbent assay (ELISA) were evaluated using 84 samples submitted to different pre-analytical conditions. FINDINGS Sensitivity and negative predictive value were directly affected by sample storage conditions. RT-PCR and virus isolation showed greater dependence on well-conserved samples for an accurate diagnosis. Interestingly, even storage at -30ºC for a relatively short time (15 days) was not adequate for accurate results using virus isolation and RT-PCR tests. On the other hand, NS1 ELISA showed no significant reduction in positivity for aliquots tested under the same conditions as in the previous tests. MAIN CONCLUSIONS Our results support the stability of the NS1 marker in ELISA diagnosis and indicate that the accuracy of routine tests such as virus isolation and RT-PCR is significantly affected by inadequate transport and storage conditions of serum samples |
| Disciplinas: | Medicina |
| Palabras clave: | Diagnóstico, Microbiología, Dengue, ARN, Técnicas de diagnóstico, Muestras séricas, Condiciones de almacenamiento |
| Keyword: | Diagnosis, Microbiology, Diagnostic techniques, Dengue, RNA, Serum samples, Storage conditions |
| Texto completo: | Texto completo (Ver HTML) Texto completo (Ver PDF) |
