| Revista: | Memorias do Instituto Oswaldo Cruz |
| Base de datos: | PERIÓDICA |
| Número de sistema: | 000452401 |
| ISSN: | 0074-0276 |
| Autores: | Mezzano, Luciana1 Morán, Joana Paola1 Moreira Espinoza, María José1 Triquell, María Fernanda1 Mezzano, Julieta2 Díaz Luján, Cintia María1 Fretes, Ricardo Emilio1 |
| Instituciones: | 1Universidad Nacional de Córdoba, Instituto de Investigación en Ciencias de la Salud, Córdoba. Argentina 2Tufts University, Friedman School of Nutrition Science and Policy, Boston, Massachusetts. Estados Unidos de América |
| Año: | 2022 |
| Volumen: | 117 |
| País: | Brasil |
| Idioma: | Inglés |
| Tipo de documento: | Artículo |
| Enfoque: | Analítico, descriptivo |
| Resumen en inglés | BACKGROUND Trypanosoma cruzi crosses the placental barrier and produces the congenital transmission of Chagas disease (CD). Structural alterations of the chorionic villi by this parasite have been described in vitro, but little is known about trophoblast turnover in placentas from women with CD. OBJECTIVE To analyze the proliferation and fusion processes in placentas from women with CD. METHODS Archived human term placenta paraffin-embedded blocks were used, from women with CD (CDP), and no pathology (NP). Immunohistochemistry tests were performed for Ki67 to calculate the proliferation index (PI) of cytotrophoblast (CTB) and Syncytin-1, a fusion marker of syncytiotrophoblast (STB). Hematoxylin/Eosin stained sections were employed to analyze STB percentages, STB detachment areas and syncytial knots quantity. Non parametric Student’s t-tests were performed (p < 0.05). RESULTS Syncytial knots and STB detachment significantly increased in placental villi from the CDP group. STB percentage was significantly lower in the CDP group as well as the PI and Syncytin-1 expression significantly decreased in these placentas, compared with control (NP). CONCLUSION Dynamic of trophoblast turnover is altered in placentas from women with CD. These changes may lead into a gap in the placental barrier possibly allowing the parasite entry into the chorionic villi |
| Disciplinas: | Medicina |
| Palabras clave: | Parasitología, Enfermedad de Chagas, Enfermedades congénitas, Mecanismos de infección, Trofoblastos, Trypanosoma cruzi |
| Keyword: | Parasitology, Chagas disease, Congenital diseases, Infection mechanisms, Trypanosoma cruzi, Trophoblasts |
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