A non-hepatotropic parasite infection increases mortality in the acetaminophen-induced acute liver failure murine model: possible roles for IL-5 and IL-6
Título del documento: A non-hepatotropic parasite infection increases mortality in the acetaminophen-induced acute liver failure murine model: possible roles for IL-5 and IL-6
Revista: Memorias do Instituto Oswaldo Cruz
Base de datos: PERIÓDICA
Número de sistema: 000401455
ISSN: 0074-0276
Autores: 1
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Instituciones: 1Centro de Investigación Científica y de Educación Superior de Ensenada, Departamento de Innovación Biomédica, Ensenada, Baja California. México
2Universidad Nacional Autónoma de México, Facultad de Medicina, Ciudad de México. México
3Universidad Nacional Autónoma de México, Facultad de Estudios Superiores Iztacala, Los Reyes Iztacala, Estado de México. México
4Universidad Nacional Autónoma de México, Instituto de Investigaciones Biomédicas, Ciudad de México. México
5Instituto Nacional de Cardiología Ignacio Chávez, Departamento de Biología Molecular, Ciudad de México. México
Año:
Periodo: Dic
Volumen: 111
Número: 12
Paginación: 757-764
País: Brasil
Idioma: Inglés
Tipo de documento: Artículo
Enfoque: Experimental, aplicado
Resumen en inglés We evaluated the effects of a non-hepatotropic parasite infection (Taenia crassiceps) on the outcome of acetaminophen-induced acute liver failure in mice. Uninfected and T. crassiceps infected mice orally received either 300 mg/kg acetaminophen or water as vehicle (n = 5 per group). Survival analysis, hepatocyte necrosis, alanine aminotransferase (ALT) levels, CYP2E1 protein, interleukin (IL-) 5, and IL-6 were assessed for all groups. All infected mice died within 16 h after exposure to acetaminophen (Tc+APAP group), whereas only one-third of uninfected animals exposed to acetaminophen (APAP group) died. Uninfected (Control group) and infected (Tc group) mice that received the vehicle showed no liver damage. Tc+APAP mice exhibited massive liver necrosis characterised by marked balloning degeneration of hepatocytes and higher serum ALT compared to Control, Tc, and APAP animals. Liver tissue from Tc+APAP mice also displayed increased expression of CYP2E1 protein and higher mRNA and protein levels of IL-5 and IL-6 compared to the other groups. These findings suggest that non-hepatotropic parasite infections may increase mortality following acute liver failure by promoting hepatocyte necrosis via IL-5 and IL-6-dependent CYP2E1 overproduction. This study identifies new potential risk factors associated with severe acute liver failure in patients
Disciplinas: Medicina
Palabras clave: Gastroenterología,
Medicina experimental,
Parasitología,
Enfermedad hepática,
Falla hepática aguda,
Infecciones parasitarias,
Taenia crassiceps,
Acetaminofén,
Interleucina
Keyword: Medicine,
Experimental medicine,
Gastroenterology,
Parasitology,
Liver diseases,
Acute liver failure,
Parasitic infection,
Taenia crassiceps,
Acetaminophen,
Interleukin
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