| Revista: | Jornal brasileiro de patologia e medicina laboratorial |
| Base de datos: | PERIÓDICA |
| Número de sistema: | 000297293 |
| ISSN: | 1676-2444 |
| Autores: | Thomazini, Cristiane Melissa1 Pinheiro, Nídia Alice Pardini, Maria Ines Naresse, Luis Eduardo Rodrigues, Maria Aparecida Marchesan |
| Instituciones: | 1Universidade Estadual Paulista "Julio de Mesquita Filho", Faculdade de Medicina, Botucatu, Sao Paulo. Brasil |
| Año: | 2006 |
| Periodo: | Feb |
| Volumen: | 42 |
| Número: | 1 |
| Paginación: | 25-30 |
| País: | Brasil |
| Idioma: | Portugués |
| Tipo de documento: | Artículo |
| Enfoque: | Caso clínico, analítico |
| Resumen en inglés | BACKGROUND: The rates of Helicobacter pylori infection are very high worldwide, but only a minority of infected patients develop gastric carcinoma. This might be related, among several factors, to the colonization of the human stomach by pathogenic Helicobacter pylori strains. OBJECTIVE: To investigate the distribution of cagA and vacA genotypes of Helicobacter pylori in paraffin-embedded gastric samples from patients with gastric cancer. MATERIAL AND METHODS: Paraffin-embedded samples from 42 patients with gastric cancer were histologically examined and evaluated by PCR for H. pylori cagA and vacA (s and m regions) genotypes. RESULTS: Histological analysis allowed direct visualization of H. pylori in 85.7% of cases and PCR for urease C gene detected H. pylori in 95% of cases. The presence of cagA gene was detected in 23 (54.7%) patients with gastric cancer. The s1 allele from vacA gene was found in samples from 24 (57.1%) patients and the m1 allele in 26 (61.9 %). The s1m1 genotype was detected in 24 (57.1%) patients with gastric cancer. The s2 allele was found in samples from four patients (9.5%) and the m2 allele in three (7.1%) patients. The distribution of H. pylori genotypes was similar in both intestinal and diffuse types of gastric carcinoma. CONCLUSION: Our results confirm the relevance of the pathogenic cagA and vacA H. pylori genotypes for significant organic lesions, such as gastric cancer, suggesting a possible role for H. pylori in the pathogenesis of gastric carcinoma |
| Resumen en portugués | CONCLUSÕES: Os resultados confirmam a relevância dos genótipos patogênicos cagA e vacA do H. pylori para lesões orgânicas significativas tais como o câncer gástrico, sugerindo a participação dessa bactéria na cadeia de eventos da oncogênese gástrica |
| Disciplinas: | Medicina |
| Palabras clave: | Gastroenterología, Oncología, Helicobacter pylori, Cáncer gástrico, CagA, VacA |
| Keyword: | Medicine, Gastroenterology, Oncology, Helicobacter pylori, Gastric cancer, CagA, VacA |
| Texto completo: | Texto completo (Ver HTML) |
