| Revista: | International braz j urol |
| Base de datos: | PERIÓDICA |
| Número de sistema: | 000434726 |
| ISSN: | 1677-5538 |
| Autores: | Corrêa, Lanna Beatriz Neves Silva1 Costa, Carlos Alberto Soares da2 Ribas, José Antônio Silva3 Boaventura, Gilson Teles2 Chagas, Mauricio Alves1 |
| Instituciones: | 1Universidade Federal Fluminense, Laboratorio de Biomorfologia Celular e Extracelular, Niteroi, Rio de Janeiro. Brasil 2Universidade Federal Fluminense, Departamento de Nutricao e Dietetica, Nutricao Experimental, Niteroi, Rio de Janeiro. Brasil 3Universidade Federal Fluminense, Departamento de Fisiologia e Farmacologia, Niteroi, Rio de Janeiro. Brasil |
| Año: | 2019 |
| Periodo: | Jul-Ago |
| Volumen: | 45 |
| Número: | 4 |
| País: | Brasil |
| Idioma: | Inglés |
| Tipo de documento: | Artículo |
| Enfoque: | Experimental, aplicado |
| Resumen en inglés | Introduction Chronic hyperglycemia is caused by diabetes mellitus-committed genital morphophysiology, and oxidative stress is one of the main factors involved in this process. Alpha lipoic acid (ALA) can prevent metabolic and morphological changes in diabetic individuals. Objectives In present study, we evaluated the effects of regular ALA consumption on the spermatogenesis and histoarchitecture in the male genital system of diabetic rats. Materials and Methods Thirty-two Wistar rats were divided into groups: Control (CG); Diabetic Control (DCG), receiving commercial diet: ALA Group (ALAG) and Diabetic ALA Group (DALAG), fed diets with added ALA (300 mg/Kg bw). The diabetic groups received a single injection of streptozotocin (60 mg/kg). After sixty days of the diet, the animals were euthanized, and semen, testis and epididymis samples were collected. A histomorphometric analysis was performed to determine the epithelial height, tubular and luminal diameter, tubular and luminal area of seminiferous tubules and each epididymal region. Sertoli cells were evidenced using the antivimenti antibody and were quantified. The results were statistically analyzed by the ANOVA test. Results At the end of the experiment, the DALAG glycemia was significantly lower than DCG. The histomorphometric parameters of the seminiferous and epididymal tubules did not show improvement in the DALAG. However, there was an improvement in the DALAG in terms of the concentration, motility and percentage of spermatic pathologies, as well as in the number of Sertoli cells (p<0.001). Conclusions The results demonstrated that supplementation with the ALA antioxidant retards testicular lesions and preserve the process of spermatogenesis in diabetes |
| Disciplinas: | Medicina |
| Palabras clave: | Medicina experimental, Farmacología, Endocrinología, Diabetes, Acido alfa lipoico, Actividad antioxidante, Testículos, Epidídimo, Análisis histológico, Modelos animales |
| Keyword: | Experimental medicine, Pharmacology, Endocrinology, Diabetes, Alpha lipoic acid, Antioxidant activity, Testis, Epididymis, Histological analysis, Animal models |
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