| Revista: | Genetics and molecular biology |
| Base de datos: | PERIÓDICA |
| Número de sistema: | 000419990 |
| ISSN: | 1415-4757 |
| Autores: | Xiao, Na1 Tang, Yi-Tong1 Li, Zhi-Shan2 Cao, Rui3 Wang, Rong2 Zou, Jiu-Ming2 Pei, Jiao2 |
| Instituciones: | 1Hubei University of Arts and Science, Medical College, Xianyang, Shaanxi. China 2Hubei University of Arts and Science, Department of Clinical Laboratory, Xianyang, Shaanxi. China 3Maternal and Child Health Care Hospital of Dongguan, Department of Internal Medicine, Dongguan. China |
| Año: | 2018 |
| Periodo: | Sep |
| Volumen: | 41 |
| Número: | 3 |
| Paginación: | 555-561 |
| País: | Brasil |
| Idioma: | Inglés |
| Tipo de documento: | Artículo |
| Enfoque: | Experimental |
| Resumen en inglés | This study aimed to develop a simple and rapid method to detect KRAS gene mutations for conventional clinical applications under laboratory conditions. The genotype of mutation sites was determined based on the occurrence of target bands in the corresponding lanes of the reaction tubes through polymerization-conjunction of the probes, probe purification and amplification, and agarose gel electrophoresis. Circulating DNA samples were obtained from the plasma of 72 patients with lung cancer, which were identified based on six mutation sites (G12S, G12R, G12C, G12D, G12A, and G12V) of codon 12 of the KRAS gene. The detection results were compared with direct sequenc- ing data. The proposed detection method is characterized by simple operation, high specificity, and high sensitivity (2%). This method can detect the mutations of three samples at G12S, G12R, and G12A. In the direct sequencing spectra of these samples, the genotype could not be determined due to the lack of evident sequencing peaks that correspond to the basic group of mutations. In conclusion, a simple and rapid method was established based on probe polymerization-conjunction-agarose gel electrophoresis for detecting KRAS gene mutations. This method can be applied to the conventional mutation detection of inhomogeneous samples |
| Disciplinas: | Medicina, Química |
| Palabras clave: | Genética, Bioquímica, Mutación, ADN circulante, Reacción de polimerización-conjunción, Electroforesis, Gel de agarosa, Gen K-ras |
| Keyword: | Genetics, Biochemistry, Mutation, Circulating DNA, Polymerization-conjunction reaction, Electrophoresis, Agarose gel, K-ras gene |
| Texto completo: | Texto completo (Ver PDF) |
