MiR-137-mediated negative relationship between LGR4 and RANKL modulated osteogenic differentiation of human adipose-derived mesenchymal stem cells
Título del documento: MiR-137-mediated negative relationship between LGR4 and RANKL modulated osteogenic differentiation of human adipose-derived mesenchymal stem cells
Revista: Genetics and molecular biology
Base de datos: PERIÓDICA
Número de sistema: 000459264
ISSN: 1415-4757
Autores: 1
2
Instituciones: 1Peking University School, Department of General Dentistry II, Beijing. China
2Health Service Department of the Guard Bureau of the General Office of the Central Committee of the Communist Party of China, Beijing. China
Año:
Volumen: 45
Número: 3
País: Brasil
Idioma: Inglés
Tipo de documento: Artículo
Enfoque: Experimental, analítico
Resumen en inglés MicroRNA-137 (miR-137) has recently emerged as an osteogenic regulator in several cell lines. This study aimed to identify the function of miR-137 on the crosstalk between leucine rich repeat containing G protein-coupled receptor 4 (LGR4) and receptor activator of nuclear factor-κB ligand (RANKL), thus unveiling the critical role of LGR4-RANKL interplay in the osteogenic differentiation of human adipose-derived mesenchymal stem cells (hASCs). By examining the osteogenic capacity and possible downstream genes expression with miR-137 overexpression/knockdown, we found that miR-137 downregulated LGR4 while upregulating RANKL. According to the results of dual-luciferase reporter assay, LGR4 was validated as a direct target of miR-137. Surprisingly, a negative relationship between LGR4 and RANKL was confirmed by the knockdown of these two genes. Furthermore, RANKL inhibitor could alleviate or reverse the inhibitory effects on osteogenesis generated by LGR4 knockdown. Collectively, this study indicated that miR-137-induced a negative crosstalk between LGR4 and RANKL that could contribute to the osteogenic regulation of hASCs and provide more systematic and in-depth understanding of epigenetic modulation by miR-137
Disciplinas: Biología,
Medicina
Palabras clave: Medicina experimental,
Genética,
Relación negativa mediada por MiR-137 entre LGR4 y RANKL modulación osteogénica de células madre mesenquimales derivadas de tejido adiposo humano,
MicroARNs,
LGR4,
Tejido adiposo,
Células madre mesenquimales,
RANKL
Keyword: Experimental medicine,
Genetics,
RANKL,
MicroRNAs,
LGR4,
Adipose tissue,
Mesenchymal stem cells
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