| Revista: | Genetics and molecular biology |
| Base de datos: | PERIÓDICA |
| Número de sistema: | 000401320 |
| ISSN: | 1415-4757 |
| Autores: | Poersch-Bortolon, Liane Balvedi1 Pereira, Jorge Fernando2 Nhani-Junior, Antonio2 Soto Gonzáles, Hebert Hernán2 Torres, Gisele Abigail Montan2 Consoli, Luciano2 Arenhart, Rafael Augusto1 Bodanese-Zanettini, Maria Helena1 Margis-Pinheiro, Marcia1 |
| Instituciones: | 1Universidade Federal do Rio Grande do Sul, Instituto de Biociencias, Porto Alegre, Rio Grande do Sul. Brasil 2Empresa Brasileira de Pesquisa Agropecuaria, Trigo, Passo Fundo, Rio Grande do Sul. Brasil |
| Año: | 2016 |
| Periodo: | Dic |
| Volumen: | 39 |
| Número: | 4 |
| Paginación: | 629-645 |
| País: | Brasil |
| Idioma: | Inglés |
| Tipo de documento: | Artículo |
| Enfoque: | Experimental, aplicado |
| Resumen en inglés | Drought limits wheat production in the Brazilian Cerrado biome. In order to search for candidate genes associated to the response to water deficit, we analyzed the gene expression profiles, under severe drought stress, in roots and leaves of the cultivar MGS1 Aliança, a well-adapted cultivar to the Cerrado. A set of 4,422 candidate genes was found in roots and leaves. The number of down-regulated transcripts in roots was higher than the up-regulated transcripts, while the opposite occurred in leaves. The number of common transcripts between the two tissues was 1,249, while 2,124 were specific to roots and 1,049 specific to leaves. Quantitative RT-PCR analysis revealed a 0.78 correlation with the expression data. The candidate genes were distributed across all chromosomes and component genomes, but a greater number was mapped on the B genome, particularly on chromosomes 3B, 5B and 2B. When considering both tissues, 116 different pathways were induced. One common pathway, among the top three activated pathways in both tissues, was starch and sucrose metabolism. These results pave the way for future marker development and selection of important genes and are useful for understanding the metabolic pathways involved in wheat drought response |
| Disciplinas: | Biología |
| Palabras clave: | Fisiología vegetal, Genética, Déficit hídrico, Resistencia a la sequía, Expresión génica, Genes candidatos, Triticum aestivum, Gramíneas |
| Keyword: | Biology, Genetics, Plant physiology, Water deficit, Drought resistance, Gene expression, Candidate gene, Triticum aestivum, Gramineae |
| Texto completo: | Texto completo (Ver HTML) |
