| Revista: | Genetics and molecular biology |
| Base de datos: | PERIÓDICA |
| Número de sistema: | 000424581 |
| ISSN: | 1415-4757 |
| Autores: | Medel-Flores, Olivia1 Valenzuela-Rodríguez, Vania Alejandra;1 Ocadiz-Delgado, Rodolfo3 Castro-Muñoz, Leonardo Josué1 Hernández-Leyva, Sandra2 Lara-Hernández, Gabriel2 Silva Escobedo, Jesús Gabriel2 Vidal, Patricio Gariglio3 Sánchez Monroy, Virginia1 |
| Instituciones: | 1Instituto Politécnico Nacional, Escuela Nacional de Medicina y Homeopatía, Ciudad de México. México 2Secretaría dela Defensa Nacional, Laboratorio Multidisciplinario de Investigación, Ciudad de México. México 3Instituto Politécnico Nacional, Centro de Investigación y de Estudios Avanzados, Ciudad de México. México |
| Año: | 2018 |
| Periodo: | Dic |
| Volumen: | 41 |
| Número: | 4 |
| Paginación: | 781-789 |
| País: | Brasil |
| Idioma: | Inglés |
| Tipo de documento: | Artículo |
| Enfoque: | Caso clínico |
| Resumen en inglés | Abstract The aim of this study was to evaluate the association between prostate cancer (PCa) and Human papilloma virus(HPV) infection in the Mexican population. We studied 356 paraffin-embedded tissues from unrelated Mexican menwith PCa or benign prostatic hyperplasia (BPH), with the latter serving as control. HPV detection was performed bypolymerase chain reaction (PCR) using universal primers, and viral genotypes were detected using sequencing ormultiplex PCR. Light microscopy analyses enabled the identification of koilocytes in samples subsequently analyzedfor HPV detection byin situPCR and for p16-INK4A expression by immunohistochemistry. The results showed thathigh risk- (HR) HPVs were detected in 37/189 (19.6%) PCa specimens compared to 16/167 (9.6%) of BHP speci-mens (odds ratio 2.3; 95% CI= 1.2 to 4.3;p=0.01). These data suggest HR-HPV may play a role in PCa. HPV 52 and58 were the most frequent genotypes (33 and 17%, respectively) detected in the population studied. Koilocytes weredetected in allin situPCR-HPV-positive samples, representing a pathognomonic feature of infection, and we ob-served the overexpression of p16-INK4A in HPV-positive samples compared to HPV-negative samples, indirectlysuggesting the presence of HR-HPV E7 oncoprotein. These results suggest that HPV infection plays an importantrole in prostate cancer development |
| Disciplinas: | Biología, Medicina, Química |
| Palabras clave: | Virus, Anatomía e histología, Bioquímica, HPV, Próstata, Cáncer, Coilocitos |
| Keyword: | Virus, Anatomy and histology, Biochemistry, HPV, Prostate, Cancer, Koilocytes |
| Texto completo: | Texto completo (Ver PDF) |
