| Revista: | Genetics and molecular biology |
| Base de datos: | PERIÓDICA |
| Número de sistema: | 000347653 |
| ISSN: | 1415-4757 |
| Autores: | Khouri, Juliana1 Resck, Ines Sabioni2 Pocas-Fonseca, Marcio1 Sousa, Thiago M.M1 Pereira, Luciana O1 Oliveira, Adriano B.B1 Grisolia, Cesar Koppe1 |
| Instituciones: | 1Universidade de Brasilia, Instituto de Ciencias Biologicas, Brasilia, Distrito Federal. Brasil 2Universidade de Brasilia, Instituto de Quimica, Brasilia, Distrito Federal. Brasil |
| Año: | 2007 |
| Periodo: | Jun |
| Volumen: | 30 |
| Número: | 2 |
| Paginación: | 442-448 |
| País: | Brasil |
| Idioma: | Inglés |
| Tipo de documento: | Artículo |
| Enfoque: | Experimental |
| Resumen en inglés | The effectiveness of an aqueous extract of Caryocar brasiliense (Caryocaraceae) Camb pulp, popularly known in Brazil as pequi, against clastogenicity induced by cyclophosphamide and bleomycin was evaluated using an in vivo mouse bone marrow cell micronuclei test, an in vitro Chinese hamster ovary cell (CHO-K1) chromosome aberration test and an in vitro antioxidant assay based on the oxidative damage to 2-deoxy-D-ribose (2-DR) induced by hydroxyl radicals (•OH) generated by the reaction between ascorbic acid and (Fe III)-EDTA. In mouse bone marrow cells the extract showed a protective effect against micronuclei induced by cyclophosphamide and bleomycin but did not interfere with polychromatic bone marrow erythrocyte proliferation, except when the mice had been treated with the highest dose of cyclophosphamide. When CHO-K1 cells were pretreated by adding 0.01, 0.05 or 0.1 mL of extract per mL of cell culture medium 24 or 48 h before bleomycin or cyclophosphamide there was a protective effect against chromosome breaks and a significant decrease in the mitotic index (a measure of cytotoxicity) of the CHO-K1 cells. The extract also had a protective effect against oxidative hydroxyl radical damage to 2-DR. This study suggests that C. brasiliense pulp aqueous extract has anticlastogenic potential, possibly due to its antioxidative properties |
| Disciplinas: | Agrociencias, Química, Biología |
| Palabras clave: | Frutales, Bioquímica, Biología celular, Caryocar brasiliense, Anticlastogenecidad, Antioxidantes, Micronúcleos |
| Keyword: | Agricultural sciences, Chemistry, Biology, Fruit trees, Biochemistry, Cell biology, Caryocar brasiliense, Anticlastogenicity, Antioxidants, Micronucleus |
| Texto completo: | Texto completo (Ver PDF) |
