Revista: | Gaceta médica de Caracas |
Base de datos: | |
Número de sistema: | 000595940 |
ISSN: | 2739-0012 |
Autores: | Reza Arifianto, Muhammad1 Suroto, Heri2 Al Fauzi, Asra1 Arifin Parenrengi, Muhammad1 Utomo, Budi3 Hafid Bajamal, Abdul1 |
Instituciones: | 1Department of Neurosurgery Dr. Soetomo General Academic Hospital, Surabaya, East Java, Indonesia. Faculty of Medicine - Universitas Airlangga, Surabaya, East Java, Indonesia, 2Department of Orthopaedics & Traumatology Dr. Soetomo General Academic Hospital, Surabaya, East Java, Indonesia. Faculty of Medicine - Universitas Airlangga, Surabaya, East Java, Indonesia. Cell & Tissue Bank Regenerative Medicine, Dr. Soetomo General Academic Hospital, Surabaya, Indonesia., 3Faculty of Medicine - Universitas Airlangga, Surabaya, East Java, Indonesia, |
Año: | 2023 |
Volumen: | 131 |
Número: | 4 |
País: | Venezuela |
Idioma: | Español |
Resumen en español | Objective: This study aimed to conduct a cytotoxicity test in determining the viability and proliferation profile for novel material of silicone rubber/polyvinyl alcohol (PVA) with or without Zirconium oxide on mesenchymal stromal cell culture. Methods: An in vitro study was carried out on adipose-derived mesenchymal stromal cell culture. Samples were divided into five groups: control, silicone rubber/polyvinyl alcohol (PVA) without Zirconium oxide, silicone rubber/polyvinyl alcohol (PVA) with Zirconium oxide 1 %, 3 %, and 5 %. Each group contained 2105 seeded MSCs/well stained with MTT for its viability. For proliferation, MTT staining was performed on days 1, 3, and 5 to assess the trend of the percentage of the living cell. Statistical analysis was conducted using ANOVA, or the Kruskal-Wallis test with a CI of 95%. |
Palabras clave: | Silicone rubber, Polyvinyl alcohol (PVA), Zirconium oxide (ZrO2), Cytotoxicity, MTT assay |
Texto completo: | Texto completo (Ver PDF) Texto completo (Ver HTML) |