Effect of Silicone Rubber/Polyvinyl Alcohol /Zirconium Oxide Compound on Mesenchymal Stromal Cells



Título del documento: Effect of Silicone Rubber/Polyvinyl Alcohol /Zirconium Oxide Compound on Mesenchymal Stromal Cells
Revista: Gaceta médica de Caracas
Base de datos:
Número de sistema: 000595940
ISSN: 2739-0012
Autores: 1
2
1
1
3
1
Instituciones: 1Department of Neurosurgery Dr. Soetomo General Academic Hospital, Surabaya, East Java, Indonesia. Faculty of Medicine - Universitas Airlangga, Surabaya, East Java, Indonesia,
2Department of Orthopaedics & Traumatology Dr. Soetomo General Academic Hospital, Surabaya, East Java, Indonesia. Faculty of Medicine - Universitas Airlangga, Surabaya, East Java, Indonesia. Cell & Tissue Bank Regenerative Medicine, Dr. Soetomo General Academic Hospital, Surabaya, Indonesia.,
3Faculty of Medicine - Universitas Airlangga, Surabaya, East Java, Indonesia,
Año:
Volumen: 131
Número: 4
País: Venezuela
Idioma: Español
Resumen en español Objective: This study aimed to conduct a cytotoxicity test in determining the viability and proliferation profile for novel material of silicone rubber/polyvinyl alcohol (PVA) with or without Zirconium oxide on mesenchymal stromal cell culture. Methods: An in vitro study was carried out on adipose-derived mesenchymal stromal cell culture. Samples were divided into five groups: control, silicone rubber/polyvinyl alcohol (PVA) without Zirconium oxide, silicone rubber/polyvinyl alcohol (PVA) with Zirconium oxide 1 %, 3 %, and 5 %. Each group contained 2105 seeded MSCs/well stained with MTT for its viability. For proliferation, MTT staining was performed on days 1, 3, and 5 to assess the trend of the percentage of the living cell. Statistical analysis was conducted using ANOVA, or the Kruskal-Wallis test with a CI of 95%.
Palabras clave: Silicone rubber,
Polyvinyl alcohol (PVA),
Zirconium oxide (ZrO2),
Cytotoxicity,
MTT assay
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