| Revista: | Electronic journal of biotechnology |
| Base de datos: | PERIÓDICA |
| Número de sistema: | 000360052 |
| ISSN: | 0717-3458 |
| Autores: | Federici, María Teresa1 Marcondes, Jackson A2 Picchi, Simone Cristina6 Stuchi, Eduardo Sanches3 Fadel, André Luiz4 Laia, Marcelo L5 Lemos, Manoel Victor Franco2 Lemos, Eliana Gertrudes Macedo2 |
| Instituciones: | 1Instituto Nacional de Investigación Agropecuaria, Estación Experimental Wilson Ferreira Aldunate, Canelones. Uruguay 2Universidade Estadual Paulista "Julio de Mesquita Filho", Faculdade de Ciencias Agrarias e Veterinarias, Jaboticabal, Sao Paulo. Brasil 3Empresa Brasileira de Pesquisa Agropecuaria, Mandioca e Fruticultura Tropical, Cruz das Almas, Bahia. Brasil 4Universidade de Sao Paulo, Escola Superior de Agricultura "Luiz de Queiroz", Piracicaba, Sao Paulo. Brasil 5Universidade Federal dos Vales do Jequitinhonha e Mucuri, Faculdade de Ciencias Agrarias, Diamantina, Minas Gerais. Brasil 6Agencia Paulista de Tecnologia dos Agronegocios, Centro de Citricultura "Sylvio Moreira", Cordeiropolis, Sao Paulo. Brasil |
| Año: | 2012 |
| Periodo: | May |
| Volumen: | 15 |
| Número: | 3 |
| Paginación: | 4-4 |
| País: | Chile |
| Idioma: | Inglés |
| Tipo de documento: | Artículo |
| Enfoque: | Experimental |
| Resumen en inglés | Xylella fastidiosa inhabits the plant xylem, a nutrient-poor environment, so that mechanisms to sense and respond to adverse environmental conditions are extremely important for bacterial survival in the plant host. Although the complete genome sequences of different Xylella strains have been determined, little is known about stress responses and gene regulation in these organisms. In this work, a DNA microarray was constructed containing 2,600 ORFs identified in the genome sequencing project of Xylella fastidiosa 9a5c strain, and used to check global gene expression differences in the bacteria when it is infecting a symptomatic and a tolerant citrus tree. Different patterns of expression were found in each variety, suggesting that bacteria are responding differentially according to each plant xylem environment. The global gene expression profile was determined and several genes related to bacterial survival in stressed conditions were found to be differentially expressed between varieties, suggesting the involvement of different strategies for adaptation to the environment. The expression pattern of some genes related to the heat shock response, toxin and detoxification processes, adaptation to atypical conditions, repair systems as well as some regulatory genes are discussed in this paper. DNA microarray proved to be a powerful technique for global transcriptome analyses. This is one of the first studies of Xylella fastidiosa gene expression in vivo which helped to increase insight into stress responses and possible bacterial survival mechanisms in the nutrient-poor environment of xylem vessels |
| Disciplinas: | Biología, Química |
| Palabras clave: | Bacterias, Genética, Bioquímica, Citrus, Microarreglos, Xylella fastidiosa |
| Keyword: | Biology, Chemistry, Bacteria, Genetics, Biochemistry, Citrus, Microarrays, Xylella fastidiosa |
| Texto completo: | Texto completo (Ver PDF) |
