| Revista: | Electronic journal of biotechnology |
| Base de datos: | PERIÓDICA |
| Número de sistema: | 000358432 |
| ISSN: | 0717-3458 |
| Autores: | Villalobos, Patricio1 Chávez, María Isabel1 Olguín, Yusser1 Sánchez, Elizabeth1 Valdés, Erika2 Galindo, Raúl3 Young, Manuel E1 |
| Instituciones: | 1Universidad Técnica Federico Santa María, Centro de Biotecnología, Valparaíso. Chile 2Universidad Técnica Federico Santa María, Departamento de Química, Valparaíso. Chile 3Universidad Técnica Federico Santa María, Departamento de Obras Civiles, Valparaíso. Chile |
| Año: | 2012 |
| Volumen: | 15 |
| Número: | 1 |
| País: | Chile |
| Idioma: | Inglés |
| Tipo de documento: | Artículo |
| Enfoque: | Experimental, aplicado |
| Resumen en inglés | The inadequate treatments given to the served waste water which are disposal to the rivers and sea coast are the major sources of faecal Microorganisms and enteric bacterial pathogens. They are among the most serious effects of water pollution bringing risks on public health. None of the current methods for detection of pathogens offer real-time on site solutions, are capable of delivering a simple visual detection signal, or can be easily instrumented as an indicator of the presence of a pathogen in water. The use of lipid vesicles incorporating Polydiacetylenes (PDAs) for the development of biosensors for real-time detection of pathogens has become an alternative, due to its potential for simple colorimetric response against harmful environmental effectors. However, its actual application in the field has been complicated because lipid vesicles are unable to respond specifically to environmental changes. In this paper, we report several experimental trials leading to improved response in the detection of flagellated pathogens in drinking water. Chromatic biomimetic membranes of TRCDA/DMPC and TRCDA/DMPC/Tryptophan were used in agar and liquid media, which were challenged with different amounts of Escherichia coli and Salmonella typhimurium. In addition, the effect of some divalent cations on the interaction with vesicles TRCDA/DMPC was investigated. The results indicated an improvement in the response times, both visually and quantitatively, through the use of TRIS-EDTA and proper growing conditions for E. coli and Salmonella. With the application of both conditions, it was possible by incubation at 35ºC to promote bacterial growth, therefore avoiding a dramatic effect on the colour change over control samples which may invalidate the test. Our experiments indicated that the minimum bacterial concentration necessary to produce the transition from blue to red on the vesicles as biosensor approaches 108 CFU/ml within 4 hrs, faster than traditional metho |
| Disciplinas: | Biología |
| Palabras clave: | Biotecnología, Microbiología, Agua potable, Análisis microbiológico, Biosensores, Vesículas lipídicas, Detección de bacterias, Colorimetría |
| Keyword: | Biology, Biotechnology, Microbiology, Drinking water, Microbiological analysis, Biosensors, Lipid vesicles, Bacterial detection, Colorimetry |
| Texto completo: | Texto completo (Ver HTML) |
