| Revista: | Electronic journal of biotechnology |
| Base de datos: | PERIÓDICA |
| Número de sistema: | 000358442 |
| ISSN: | 0717-3458 |
| Autores: | Díaz, Mauricio1 Polanco, Victor2 Ramírez, Ingrid3 Peña Cortés, Hugo3 |
| Instituciones: | 1Pontificia Universidad Católica de Valparaíso, Escuela de Ingeniería Bioquímica, Valparaíso. Chile 2Universidad Mayor, Instituto de Biotecnología, Santiago de Chile. Chile 3Universidad Técnica Federico Santa María, Centro de Biotecnología Daniel Alkalay Lowitt, Valparaíso. Chile |
| Año: | 2012 |
| Volumen: | 15 |
| Número: | 1 |
| País: | Chile |
| Idioma: | Inglés |
| Tipo de documento: | Artículo |
| Enfoque: | Experimental, aplicado |
| Resumen en inglés | Jasmonic acid (JA) and 12-oxophytodienoic acid (OPDA) are signal molecules involved in the stress and defense responses in plants. A full-length cDNA clon of OPR3 encoding 12-oxophytodienoate reductase 3, key enzyme involved in the biosynthesis of JA from linolenic acid was obtained from a Solanum tuberosum cDNA library. Sequence analysis showed that OPR3 encoded a polypeptide of 400 amino acids with a predicted molecular mass of 43.9 kDa and pI of 7.72. The deduced amino acid sequence of OPR3 showed high similarities to other 12-oxophytodienoate reductases. A peroxisomal signal sequence indicates OPR3 probable location in peroxisome. Levels of OPR3 mRNA accumulated in potato leaves reaching maximum levels within 1 hr of mechanical wounding. Elevated levels of JA were correlated to expression of the OPR3 gene |
| Disciplinas: | Biología |
| Palabras clave: | Fisiología vegetal, Genética, Mecanismos de defensa vegetal, Acido jasmónico, 12-oxofitodienoato reductasa, Clonación, Solanum tuberosum |
| Keyword: | Biology, Genetics, Plant physiology, Plant defense mechanisms, Jasmonic acid, 12-oxophytodienoate reductase, Cloning, Solanum tuberosum |
| Texto completo: | Texto completo (Ver HTML) |
