| Revista: | Electronic journal of biotechnology |
| Base de datos: | PERIÓDICA |
| Número de sistema: | 000398055 |
| ISSN: | 0717-3458 |
| Autores: | Postiglioni, Alicia1 García, Cristina B2 Rincón, Gonzalo3 Arruga, M. Victoria2 |
| Instituciones: | 1Universidad de la República, Facultad de Veterinaria, Montevideo. Uruguay 2Universidad de Zaragoza, Facultad de Veterinaria, Zaragoza. España 3University of California, Department of Animal Science, Davis, California. Estados Unidos de América |
| Año: | 2011 |
| Volumen: | 14 |
| Número: | 3 |
| Paginación: | 1-5 |
| País: | Chile |
| Idioma: | Inglés |
| Tipo de documento: | Nota breve o noticia |
| Enfoque: | Analítico, descriptivo |
| Resumen en inglés | Robertsonian translocation (rob(1;29)) is the most frequent structural chromosomal abnormality in cattle. Heterozygous carriers have a normal phenotype but show a 3- 5% decrease in fertility. Chromatin decondensation was evaluated similar to the inactive X chromosome when submitted to demethylating agent. Based on this result, and the concept that imprinted genes are essential in embryonic development, we decided to query genes located on BTA1 and BTA29 that could undergo genome imprinting. The c ollagen typeVIII - α 1 (Col8A 1 ) acted on extracellular matrix structural proteins. DNA bisulfite conversion and sequentiation methods were used to compare its differential methylation patterns. It was performed on eight Creole cattle DNA blood samples from normal and rob(1;29) carriers. An in silico screening for CpG islands in its promoter uncovered a single region of 454 bp prone to methylation. BiQ -Analizer software was used to show the selective conversion of unmethylated cytosines to uracils obtaining the following results: unmethylated CpGs: 0.000 (0 cases), methylated CpGs: 0.802 (77 cases) and CpGs not present: 0.198 (19 cases). No differences between samples were observed in this highly methylated region. This technique was successfully applied so it i s a straightforward methodology that can be utilized to evaluate different tissue associated to specific gene expression |
| Disciplinas: | Medicina veterinaria y zootecnia, Química, Biología |
| Palabras clave: | Bovinos, Bioquímica, Anatomía e histología, Bisulfito, Colágeno, Ganado criollo, ADN, Citosinas, Metilación |
| Keyword: | Veterinary medicine and animal husbandry, Chemistry, Biology, Bovines, Biochemistry, Anatomy and histology, Bisulfite, Collagen, Creole cattle, DNA, Cytosines, Methylation |
| Texto completo: | Texto completo (Ver PDF) |
