| Revista: | Electronic journal of biotechnology |
| Base de datos: | PERIÓDICA |
| Número de sistema: | 000359558 |
| ISSN: | 0717-3458 |
| Autores: | Zhang, Wei-Wei1 Yang, Ming-Ming2 Li, Heng-Xin2 Wang, Dun1 |
| Instituciones: | 1Northwest A&F University, College of Life Sciences, Yangling, Shaanxi. China 2Northwest A&F University, College of Animal Sciences, Yangling, Shaanxi. China |
| Año: | 2011 |
| Volumen: | 14 |
| Número: | 6 |
| Paginación: | 1-1 |
| País: | Chile |
| Idioma: | Inglés |
| Tipo de documento: | Artículo |
| Enfoque: | Experimental |
| Resumen en inglés | As a precursor, pimelic acid plays an important role in biotin biosynthesis pathway of Bacillus subtilis. Fermentations supplemented with pimelic acid could improve the production of biotin, however, with a disadvantage-high cost. So it is necessary to improve the biosynthesis of pimelic acid via genetic engineering in B. subtilis. In this study, we constructed a recombinant B. subtilis strain for improving the synthesis of pimelic acid, in which a maltose-inducible Pglv promoter was inserted into the upstream of the cistron bioI-orf2-orf3 and, meanwhile, flanked by the tandem cistrons via a single crossover event. The copy number of the integrant was amplified by high-concentration resistance screen and increased to 4-5 copies. The production of pimelic acid from multiple copies integrant was about 4 times higher than that from single copy (1017.13 μg/ml VS. 198.89 μg/ml). And when induced by maltose the production of pimelic acid was about 2 times of that under non-induction conditions (2360.73 μg/ml VS. 991.59 μg/ml). Thus, these results demonstrated that the production of pimelic acid was improved obviously through reconstructed B. subtilis. It also suggested that our expression system provided a convenient source of pimelic acid that would potentially lower the cost of production of biotin from engineered B. subtilis |
| Disciplinas: | Biología, Química |
| Palabras clave: | Bacterias, Genética, Bioquímica, Bacillus subtilis, Biosíntesis, Integración, Promotor Pglv, Acido pimélico, Solo evento de cruce, Biotecnología |
| Keyword: | Biology, Chemistry, Bacteria, Genetics, Biochemistry, Bacillus subtilis, Biosynthesis, Integration, Pglv promoter, Pimelic acid, Single crossover event, Biotechnology |
| Texto completo: | Texto completo (Ver PDF) |
