| Revista: | Electronic journal of biotechnology |
| Base de datos: | PERIÓDICA |
| Número de sistema: | 000358467 |
| ISSN: | 0717-3458 |
| Autores: | Sangachini, Elham Dasi1 Hasannia, Sadegh2 Taghdir, Majid1 Pirooznia, Nazanin1 Ghadicholaei, Kamran Khalili1 |
| Instituciones: | 1University of Guilan, Faculty of Science, Rasht. Irán 2Tarbiat Modares University, Faculty of Biological Sciences, Teherán. Irán |
| Año: | 2012 |
| Volumen: | 15 |
| Número: | 2 |
| País: | Chile |
| Idioma: | Inglés |
| Tipo de documento: | Artículo |
| Enfoque: | Experimental, aplicado |
| Resumen en inglés | Background: The elastase inhibitor α-1-antitrypsin (AAT), is a member of the serpin superfamily of protease inhibitors. AAT has a characteristic secondary structure of three-β-sheets, nine-α-helices and a reactive central loop (RCL). This protein inhibits target proteases by forming a stable complex in which the cleaved RCL is inserted into β-sheet-A of the serpin, leading to a conformational change in the AAT protein. Spontaneous polymerization and instability of AAT are challenges with regard to producing drugs against AAT-deficient diseases. Therefore, the purpose of many investigations currently is to produce drugs with lower degrees of polymerization and higher stabilities. In order to investigate the effect of the N-terminal segment (residues 1-43) on AAT structure, molecular dynamic (MD) simulation was used to study structural properties including Root-mean-square deviation (RMSD), internal motions, intramolecular non-bonded interactions and the total accessible surface area (ASA) of native and reduced AAT. These properties were compared in native and truncated AAT. Results: Theoretical studies showed no noticeable differences in the dynamic and structural properties of the two structures. These findings provided the basis for the experimental phase of the study in which sequences from the two AAT constructs were inserted into the expression vector pGAPZ and transformed into Pichia pastoris. Results showed no differences in the activities and polymerization of the two AAT constructs. Conclusions: As small-scale medicines are preferred by lung drug delivery systems, in this study AAT was designed and constructed by decreasing the number of amino acids at the N-terminal region |
| Disciplinas: | Química |
| Palabras clave: | Bioquímica, Biotecnología, Inhibidores proteicos, Síntesis química, Alfa 1-antitripsina, Elastasa, Simulación molecular |
| Keyword: | Chemistry, Biochemistry, Biotechnology, Protein inhibitors, Chemical synthesis, Alpha 1-antitrypsin, Elastase, Molecular simulation |
| Texto completo: | Texto completo (Ver HTML) |
