| Revista: | Electronic journal of biotechnology |
| Base de datos: | PERIÓDICA |
| Número de sistema: | 000358434 |
| ISSN: | 0717-3458 |
| Autores: | Zhang, Yue1 Xia, Han2 Yuan, Mei3 Zhao, Chuanzhi2 Li, Aiqin2 Wang, Xingjun1 |
| Instituciones: | 1Qingdao Agricultural University, College of Life Science, Qingdao, Shandong. China 2Shandong Academy of Agricultural Sciences, High-Tech Research Center, Jinan, Shandong. China 3Shandong Peanut Research Institute, Quingdao, Shandong. China |
| Año: | 2012 |
| Volumen: | 15 |
| Número: | 1 |
| País: | Chile |
| Idioma: | Inglés |
| Tipo de documento: | Artículo |
| Enfoque: | Experimental, aplicado |
| Resumen en inglés | Chalcone isomerase (CHI) is the key enzyme that catalyzes chalcone into (2S)-flavanol or (2S)-5-desoxidation flavanol. The full length cDNA (1050 bp) of AhCHI (Arachis hypogaea CHI gene) was cloned by large scale EST sequencing using a peanut immature seed cDNA library. Sequence analysis results indicated that it was a type I CHI gene (with the accession number JN660794). The ORF of AhCHI was 768 bp, encoding a peptide of 255 amino acids with a pI of 5.189. Sequence alignment showed that the coding region of AhCHI gene is highly conserved to compare with CHI genes from other plant species. Peanut cDNA microarray and semi-quantitative RT-PCR analysis indicated that AhCHI was highly expressed in pegs. The expression level in flower and root was higher than the expression level in stem and leaf. AhCHI was expressed in a high level in seeds with a purple seed coat, while its expression was low in seed with white seed coat |
| Disciplinas: | Biología, Agrociencias |
| Palabras clave: | Genética, Frutales, Biotecnología, Mejoramiento genético, Clonación, Expresión génica, Chalcona isomerasa, Cacahuate, Arachis hypogaea |
| Keyword: | Biology, Agricultural sciences, Genetics, Fruit trees, Biotechnology, Genetic improvement, Cloning, Gene expression, Chalcone isomerase, Peanut, Arachis hypogaea |
| Texto completo: | Texto completo (Ver HTML) |
