| Revista: | Electronic journal of biotechnology |
| Base de datos: | PERIÓDICA |
| Número de sistema: | 000336222 |
| ISSN: | 0717-3458 |
| Autores: | Yuan, Wang Jun1 Han, Yuan Ji1 Dong, Mei Fang1 Shang, Fu De1 |
| Instituciones: | 1Henan University, College of Life Science, Kaifeng, Henan. China |
| Año: | 2011 |
| Periodo: | Ene |
| Volumen: | 14 |
| Número: | 1 |
| País: | Chile |
| Idioma: | Inglés |
| Tipo de documento: | Artículo |
| Enfoque: | Experimental, aplicado |
| Resumen en inglés | This study was conducted to reveal genetic diversity among 100 Osmanthus fragrans cultivars using amplified fragment length polymorphism (AFLP) markers. Eight AFLP primer combinations produced a total of 443 polymorphic fragments with an average of 64 per primer combination. The percentage of polymorphic bands (86.81%), the resolving power (Rp) (32.71) and the PIC values (0.331) showed the efficiency of used primer combinations. The revealed AFLP makers were effective in distinguishing all the cultivars considered. Cluster analysis were performed to assess patterns of diversity among cultivars and showed the abundant genetic diversity. The overall distribution pattern of molecular variation suggested that 93.33% of the total genetic variance was within the identified groups and 6.67% of the genetic variation was among the identified groups. Our results showed that AFLP markers are useful for Osmanthus fragrans germplasm discrimination as well as for investigation of genetic diversity and variation. The information will facilitate germplasm identification, conservation and new cultivar development |
| Disciplinas: | Biología |
| Palabras clave: | Angiospermas, Genética, Diversidad genética, Marcadores moleculares, Análisis de agrupamiento, Osmanthus fragrans, Oleaceae |
| Keyword: | Biology, Angiosperms, Genetics, Genetic diversity, Molecular markers, Cluster analysis, Osmanthus fragrans, Oleaceae |
| Texto completo: | Texto completo (Ver PDF) |
