A simple ethanol wash of the tissue homogenates recovers high-quality genomic DNA from Corchorus species characterized by highly acidic and proteinaceous mucilages
Título del documento: A simple ethanol wash of the tissue homogenates recovers high-quality genomic DNA from Corchorus species characterized by highly acidic and proteinaceous mucilages
Revista: Electronic journal of biotechnology
Base de datos: PERIÓDICA
Número de sistema: 000336242
ISSN: 0717-3458
Autores: 1
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Instituciones: 1Central Research Institute for Juteand Allied Fibres, Division of Crop Improvement, Calcuta, Bengala Occidental. India
Año:
Periodo: Ene
Volumen: 14
Número: 1
País: Chile
Idioma: Inglés
Tipo de documento: Artículo
Enfoque: Experimental, aplicado
Resumen en inglés A simple Miniprep based on early elimination of highly acidic and proteinaceous mucilages through ethanol washing of the tissue homogenates has been developed for the extraction of genomic DNA from mature leaves and seeds of Corchorus spp. As compared to high cetyltrimethylammonium bromide (CTAB)-NaCl DNA extraction followed by ethanol-based removal of remnant mucilages from the DNA pellet, this simple miniprep consistently and reproducibly recovers high amounts of DNA with good spectral qualities at A260/A280 and A260/A230. The purified DNA is efficiently digested by restriction endonucleases, and is suitable for PCR amplification of nuclear microsatellites with expected allele sizes
Disciplinas: Biología,
Química
Palabras clave: Bioquímica,
Biotecnología,
ADN,
Extracción química,
Enzimas de restricción,
Microsatélites,
Digestión enzimática,
Corchorus
Keyword: Biology,
Chemistry,
Biochemistry,
Biotechnology,
DNA,
Chemical extraction,
Restriction enzymes,
Microsatellites,
Enzymatic digestion,
Corchorus
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