| Revista: | Ecletica quimica |
| Base de datos: | |
| Número de sistema: | 000552646 |
| ISSN: | 0100-4670 |
| Autores: | Franco, Juliana Metzner1 Pugine, Silvana Marina Piccoli1 Scatoline, Antônio Márcio1 de Melo, Mariza Pires1 |
| Instituciones: | 1Department of Basic Science, Faculty of Animal Science and Food Engineering (FZEA), University of São Paulo, Pirassununga, SP, Brazil, |
| Año: | 2018 |
| Volumen: | 43 |
| Número: | 3 |
| Paginación: | 19-29 |
| País: | Brasil |
| Idioma: | Inglés |
| Resumen en inglés | The aim of the present study was to evaluate in vitro antioxidant capacity of Melissa extract (ME) (Melissa officinalis L.) and its protective effect on peroxyl radical-induced oxidative damage in erythrocytes. ME used in present study was obtained by rota-evaporation of the crude extract (ethanol:water/dried leaves). Total phenolic and flavonoids contend determination, 176.8 ± 13.2 mg GAE/g dw and 26.2 ± 3.2 mg QE/g dw, respectively). Total equivalent antioxidant activities, TEAC in mg TE/g dw, were 61.4 ± 5.5 and 512.4 ± 77.2 for respective FRAP assay and DPPH• radical-scavenging. The ME acts as an antioxidant on NO and O2•-, when ME exerted a higher antioxidant action on NO scavenging to compared to the ascorbic acid (1.9 times), however, the antioxidant capacity of ME on O2•- was lower than ascorbic acid (5.6 times). The values of hemolysis inhibition from ME (IC50, 2.0 ± 0.5 mg/mL) were higher than ascorbic acid (IC50, 7.1 ± 1.8 mg/mL). Extract of Melissa was able to eliminate biological free radicals, suggesting a potential to prevent oxidative damage in vivo. In fact, the ME exerted protective action on cell membrane lysis in situ. |
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