| Revista: | Dermatología cosmética, médica y quirúrgica |
| Base de datos: | PERIÓDICA |
| Número de sistema: | 000360300 |
| ISSN: | 1665-4390 |
| Autores: | Hajar Serviansky, Tamar1 Kresch Tronik, Nicole S1 Moreno Coutiño, Gabriela1 Arenas, Roberto1 Vega Memije, María Elisa1 |
| Instituciones: | 1Secretaría de Salud, Hospital Manuel Gea González, México, Distrito Federal. México |
| Año: | 2013 |
| Periodo: | Ene-Mar |
| Volumen: | 11 |
| Número: | 1 |
| Paginación: | 13-18 |
| País: | México |
| Idioma: | Español |
| Tipo de documento: | Artículo |
| Enfoque: | Aplicado, analítico |
| Resumen en español | beneficio de esta tinción para descartar otros diagnósticos diferenciales |
| Resumen en inglés | The hematoxylin-eosin stain (HE) is a commonly used histopathology tool to analyze skin sections. However, this medium does not bind to elements such as elastic fibers or mast cells, nor does it differentiate between melanic pigments and hemosiderin. Therefore, some diagnostic procedures require special stains, including: periodic acid-Schiff (PAS), to visualize microorganisms; Gomori-Grocott (methenamine silver), for screening fungi; and the and Ziehl-Neelsen and Kinyoun stains, for actinomycetes and micobacterias.1 Of these, PAS is potentially the most useful, as it binds with basal membrane glycogen and various skin structures. Objective: To describe the usefulness of PAS stain as a diagnostic tool in dermatopathology. Methodology: Review of PAS-stained samples from a total of 1000 skin biopsies obtained in 2008 by the Department of Dermatology of the General Hospital “Dr. Manuel Gea González”. Results: The authors reviewed a total of 43 PAS-stained biopsies from 36 patients diagnosed with non-infectious (58%) and infectious (42%) dermatoses. Of the latter, infectious etiology was confirmed in 33.33% of PAS-stained samples, with further 9.3% false-negative results in spite of isolation of infectious agents in culture media (11.6%). In all, 18.6% of PAS-stained samples were positive for infectious and inflammatory lesions, such as discoid lupus erythematosus. Conclusions: PAS stain is a useful diagnostic tool for clinically suspected infectious conditions of the skin. However, due to its retrospective nature, this review cannot assess the benefits of PAS in differential diagnosis |
| Disciplinas: | Medicina |
| Palabras clave: | Dermatología, Diagnóstico, Hongos, Glicogeno, Tinción, Histopatología, Acido peryódico de Schiff |
| Keyword: | Medicine, Dermatology, Diagnosis, Fungi, Glycogen, Stain, Histopathology, Schiff periodic acid |
| Texto completo: | Texto completo (Ver HTML) |
