| Revista: | Brazilian Journal of Pharmaceutical Sciences |
| Base de datos: | PERIÓDICA |
| Número de sistema: | 000451429 |
| ISSN: | 1984-8250 |
| Autores: | Shariatnasery, Maria1 Irani, Shiva1 Soleimani, Masoud2 Goodarzi, Navid3 Dinarvand, Rassoul3 |
| Instituciones: | 1Islamic Azad University, Science and Research Branch, Teherán. Irán 2Tarbiat Modares University, School of Medical Sciences, Teherán. Irán 3Tehran University of Medical Sciences, Faculty of Pharmacy , Teherán. Irán |
| Año: | 2020 |
| Volumen: | 56 |
| País: | Brasil |
| Idioma: | Inglés |
| Tipo de documento: | Artículo |
| Enfoque: | Experimental, aplicado |
| Resumen en inglés | The functional significance of upregulation miR-34a in combination with albumin-bound paclitaxel nanoparticles in U251 glioblastoma cell line has been evaluated. The MTT assay determined that miR-34a and albumin-bound paclitaxel nanoparticles can reduce cell viability, but the combination of both factors has a stronger effect on cell viability. The application of qRT-PCR has demonstrated that the transduction of miR-34a could lead to exogenous upregulation of miR-34a level and downregulation of SURVIVIN. Moreover, treatment of U251 cells with miR-34a and nanoparticles together considerably inhibit SURVIVIN expression compared to miR-34a and nanoparticles alone. Flow cytometry showed that upon miR-34a overexpression cell cycle arrested in G1 phase, while treatment with nanoparticles increased the cell population in G2 phase. Upregulation of miR-34a along with treatment with nanoparticles elevated the number of cells arrested in G1/ G2 phases of the cell cycle. Expression of miR-34a with albumin-bound paclitaxel nanoparticles reduced cell viability, downregulated SURVIVIN and enhanced cell cycle arrest in G1/G2 phases. Thus, the upregulation of miR-34a with these nanoparticles are potential candidates therapeutic for glioblastoma cancer |
| Disciplinas: | Química, Medicina |
| Palabras clave: | Oncología, Farmacología, Nanopartículas, Albúmina, MicroARNs, Paclitaxel, Sinergismo, Proliferación celular, Glioblastoma, Terapia combinada |
| Keyword: | Oncology, Pharmacology, Nanoparticles, Albumin, MicroRNAs, Paclitaxel, Synergism, Cell proliferation, Glioblastoma, Combined therapy |
| Texto completo: | Texto completo (Ver HTML) Texto completo (Ver PDF) |
