Selective Cytotoxic effect of Probiotic, Paraprobiotic and Postbiotics of L.casei strains against Colorectal Cancer Cells: Invitro studies
Título del documento: Selective Cytotoxic effect of Probiotic, Paraprobiotic and Postbiotics of L.casei strains against Colorectal Cancer Cells: Invitro studies
Revista: Brazilian Journal of Pharmaceutical Sciences
Base de datos: PERIÓDICA
Número de sistema: 000451784
ISSN: 1984-8250
Autores: 1
2
3
4
2
Instituciones: 1Islamic Azad University, Science and Research Branch, Teherán. Irán
2Razi Vaccine and Serum Research Institute, Agriculture Research, Education and Extension Organization, Karaj. Irán
3Tehran University of Medical Sciences, School of Advanced Medical Technologies, Teherán. Irán
4Tehran University of Medical Sciences, Department of Medical Genetics, Teherán. Irán
Año:
Volumen: 58
País: Brasil
Idioma: Inglés
Tipo de documento: Artículo
Enfoque: Experimental, aplicado
Resumen en inglés This study highlights the cytotoxic effect of three L. casei strains on colorectal cell lines in invitro conditions. Different concentrations of live, heat killed (HK) and cell free supernatant (CFS) of three L.casei strains were subjected to CaCo2 and MRC5 cell lines. The viability of the treated and untreated cells was determined after 72 hrs by MTT assay, and IC50 estimated. Apoptosis was evaluated by Annexin V-propidium iodide method using flow cytometry. The live, HK and CFS of the L. casei strains showed cytotoxic effects on colorectal cell lines with significant differences. The cytotoxicity effects of live cells on CaCo2 cells were significantly higher (p˂0.01) than the HK cells. A dose dependent response was observed, as higher concentrations resulted in enhanced cytotoxicity effects. Live L.casei 1296-2cells inhibited 91% of CaCo2 cell growth, with IC50 of less than 108 cfu/ml. MRS medium and concentrations of CFS at above 20% v/v, were cytotoxic to the normal cell lines. Flow cytometry analyses of L. casei 1296-2 indicated that cytotoxicity effects on CaCo2 cells is related to apoptotic induction. Invitro studies indicate that Live and CFS of L. casei 1296-2 might be promising candidate for the control of colorectal cancers
Disciplinas: Medicina
Palabras clave: Oncología,
Farmacología,
Cáncer colorrectal,
Probióticos,
Apoptosis,
Lactobacillus casei
Keyword: Oncology,
Pharmacology,
Colorectal cancer,
Probiotics,
Apoptosis,
Lactobacillus casei
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