Purified novel and new diferuloyl glycerate related phenolic acid from Pandanus odoratissimus flowers shows antioxidant, invertase inhibition and control against diabetic foot ulcer (DFU) causing bacterial pathogens - An in vitro study to establish an effective regulation over type 2 diabetes mellitus
Título del documento: Purified novel and new diferuloyl glycerate related phenolic acid from Pandanus odoratissimus flowers shows antioxidant, invertase inhibition and control against diabetic foot ulcer (DFU) causing bacterial pathogens - An in vitro study to establish an effective regulation over type 2 diabetes mellitus
Revista: Brazilian Journal of Pharmaceutical Sciences
Base de datos: PERIÓDICA
Número de sistema: 000452091
ISSN: 1984-8250
Autores: 1
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Instituciones: 1Kumaraguru College of Technology, Department of Biotechnology, Coimbatore, Tamil Nadu. India
2Kumaraguru College of Technology, Department of Mechanical Engineering, Coimbatore, Tamil Nadu. India
3Sri Ramakrishna Hospital, Chief Plastic Surgeon, Coimbatore, Tamil Nadu. India
Año:
Volumen: 58
País: Brasil
Idioma: Inglés
Tipo de documento: Artículo
Enfoque: Experimental, aplicado
Resumen en inglés Chronic type 2 diabetes mellitus (T2DM) and its associated diseases are major concern among human population and also responsible for significant mortality rate. Hence, the present study aims to evaluate and correlate the invertase inhibition, antioxidant activity and control against DFU causing bacterial pathogens by Pandanus odoratissimus flowers. Two dimensional preparative thin layer chromatography (2D PTLC) was adopted to purify the phenolic acid component and LC-MS2 was done to predict the phenolic acid structures. Standard spectrophotometry methods were adopted to investigate the in vitro invertase inhibitory and antioxidant (CUPRAC and ABTS) activities. Agar well diffusion and broth dilution assays were used to record the antibacterial property against DFU causing pathogens isolated from clinical samples. Statistical analyses were used to validate the experiments. A new and novel diferuloyl glycerate related phenolic acid (m/z 442) purified from PTLC eluate has recorded satisfactory cupric ion reducing power (ED50= 441.4±2.5 µg), moderate ABTS radical scavenging activity (IC50= 450.3±10 µg; 32.5±1.5%), and a near moderate, in vitro, invertase mixed type inhibition (24.5±4.5%; Ki: 400 µg). Similarly, bacterial growth inhibitory kinetics has showed a significant inhibition against E. coli and S. aureus
Disciplinas: Medicina,
Química
Palabras clave: Endocrinología,
Farmacología,
Pie diabético,
Plantas medicinales,
Diferuloil glicerato,
Inhibición enzimática,
Invertasa,
Pandanus odoratissimus,
Pandanaceae,
Endocrinology,
Pharmacology
Keyword: Diabetic foot,
Medicinal plants,
Diferuloyl glycerate,
Enzyme inhibition,
Invertase,
Pandanus odoratissimus,
Pandanaceae
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