| Revista: | Brazilian Journal of Pharmaceutical Sciences |
| Base de datos: | PERIÓDICA |
| Número de sistema: | 000451894 |
| ISSN: | 1984-8250 |
| Autores: | Yu, Ting1 Song, Xiaowei2 Jin, Guangyu2 Sun, Jingxin1 Jin, Zhehao2 Quan, Jishan1 |
| Instituciones: | 1Yanbian University, College of Pharmacy, Yanbian, Jilin. China 2Yanbian University Hospital, Department of Radiology, Yanbian, Jilin. China |
| Año: | 2022 |
| Volumen: | 58 |
| País: | Brasil |
| Idioma: | Inglés |
| Tipo de documento: | Artículo |
| Enfoque: | Experimental, aplicado |
| Resumen en inglés | A cationic liposomal gene delivery system comprising DOTAP, DOPE, and cholesterol was prepared and optimized. The results showed that the liposome/DNA (LP/DNA) system had spherical morphology, with a particle size of around 150 nm and zeta potential of approximately 30 mV. Cytotoxicity experiments showed that cells treated with all of the liposome carriers- with the exception of LP1-had more than 80% viability even at a weight ratio of 30. The in vitro transfection efficiency was measured using a Promega™ Luciferase Assay System. Of the tested lipoplexes, LP2/DNA showed the highest cell transfection efficiency (at a weight ratio of 10)-which was similar to or slightly lower than that of Lipofectamine® 2000 in HeLa, A549, and SPC-A1 cell lines. After freeze-drying, the cell transfection efficiency decreased slightly (P>0.05). The cell uptake mechanism study showed that LP/DNA lipoplexes mainly entered cells via clathrin-mediated and caveolin-mediated endocytic pathways. The results confirmed that LP2 has potential for use as an effective gene carrier, and provides experimental evidence to support its further development as a safe and effective gene delivery system |
| Disciplinas: | Química |
| Palabras clave: | Química farmacéutica, Liposomas catiónicos, Liberación de genes, Liofilización |
| Keyword: | Medicinal chemistry, Cationic liposomes, Gene delivery, Lyophilization |
| Texto completo: | Texto completo (Ver HTML) Texto completo (Ver PDF) |
