| Revista: | Brazilian Journal of Pharmaceutical Sciences |
| Base de datos: | PERIÓDICA |
| Número de sistema: | 000452197 |
| ISSN: | 1984-8250 |
| Autores: | Nasar, Muhammad Qasim1 Zohra, Tanzeel1 Khalil, Ali Talha2 Ovais, Muhammad3 Ullah, Ikram1 Ayaz, Muhammad4 Zahoor, Mohammad5 Shinwari, Zabta Khan1 |
| Instituciones: | 1Quaid-i-Azam University, Department of Biotechnology, Islamabad. Pakistán 2Lady Reading Hospital, Molecular Biology and Pathology Department, Peshawar, Khyber Pakhtunkhwa. Pakistán 3National Center for Nanoscience and Technology, Center for Excellence in Nanoscience, Beijing. China 4University of Malakand, Department of Pharmacy, Khyber, Pakhtunkhwa. Pakistán 5University of Malakand, Department of Chemistry, Khyber, Pakhtunkhwa. Pakistán |
| Año: | 2022 |
| Volumen: | 58 |
| País: | Brasil |
| Idioma: | Inglés |
| Tipo de documento: | Artículo |
| Enfoque: | Experimental, aplicado |
| Resumen en inglés | Plants from genus Ephedra are commonly used by the Chinese people as folk medicine for treatment of various diseases. The current study was designed to explore the ethno-pharmacological based pharmacological potentials of Ephedra intermedia Schrenk & C.A. Mey. (E. intermedia). Plant aerial parts were extracted using ten solvent systems with increasing order of polarity. Samples were analyzed for total phenolic and flavonoid contents, HPLC-DAD analysis, antibacterial, antifungal, HepG2 cell line cytotoxicity, hemolysis and antioxidant potentials following standard procedures. Highest percent extract recovery was observed in Eth+WT (25.55 % w/w) solvent system. Flavonoid and phenolic contents were higher in chloroform and Met+WT fractions respectively. Considerable antibacterial activity was shown by Eth+Met extract against B. subtilis and K. pneumonia (MIC of 11.1μg/mL for each). Eth extract exhibited high antifungal activity against A. fumigates (15±0.31 mm DIZ). Met+WT extract showed significant cytotoxicity against HepG2 cell lines with IC50 of 13.51+0.69 μg/mL. Substantial free radical scavenging activity (74.9%) was observed for Met+Eth extract. In the current study, several solvent systems were used for more effective extraction of fractions and can be useful in the isolation of phytochemicals. Various fractions exhibited considerable antimicrobial, antioxidant and cytotoxic potentials. Biological potentials of E. intermedia signify its potential uses in microbial, cancer and degenerative disorders and thus warrant further detailed studies |
| Disciplinas: | Química |
| Palabras clave: | Farmacología, Plantas medicinales, Compuestos fenólicos, Flavonoides, Bioensayos, Ephedra intermedia, Ephedraceae |
| Keyword: | Pharmacology, Medicinal plants, Phenolic compounds, Flavonoids, Bioassays, Ephedra intermedia, Ephedraceae |
| Texto completo: | Texto completo (Ver HTML) Texto completo (Ver PDF) |
