| Revista: | Brazilian Journal of Pharmaceutical Sciences |
| Base de datos: | PERIÓDICA |
| Número de sistema: | 000451905 |
| ISSN: | 1984-8250 |
| Autores: | Bannwart, Thays Avelino1 Bersani Amado, Ciomar Aparecida2 Ames, Franciele Queiroz2 Siqueira, Vera Lúcia Dias3 Oliveira, Arildo José Braz de1 Gonçalves, Regina Aparecida Correia1 |
| Instituciones: | 1Universidade Estadual de Maringa, Departamento de Farmacia, Maringa, Parana. Brasil 2Universidade Estadual de Maringa, Departamento de Farmacologia e Terapeutica, Maringa, Parana. Brasil 3Universidade Estadual de Maringa, Departamento de Analisis Clinicos e Biomedicina, Maringa, Parana. Brasil |
| Año: | 2022 |
| Volumen: | 58 |
| País: | Brasil |
| Idioma: | Inglés |
| Tipo de documento: | Artículo |
| Enfoque: | Experimental, aplicado |
| Resumen en inglés | Exopolysaccharides (EPS) produced by Klebsiella oxytoca are of environmental, pharmaceutical, and medicinal interest. However, studies about the anti-inflammatory activity of EPS produced by this microorganism still remain limited. The aim of this study was to produce, characterize, and evaluate the anti-inflammatory activity of EPS from K. oxytoca in a pleurisy model. Colorimetric analysis revealed that precipitated crude exopolysaccharides (KEPSC) and deproteinated exopolysaccharides (KEPS) present high levels of total carbohydrates (65.57% and 62.82%, respectively). Analyses of uronic acid (7.90% in KEPSC and 6.21% in KEPS) and pyruvic acid (3.01% in KEPSC and 1.68% in KEPS) confirm that the EPS are acidic. Gas chromatography-mass spectrometry analyses demonstrated that the EPS consisted of rhamnose (29.83%), glucose (11.21%), galactose (52.45%), and mannose (6.50%). The treatment of an experimental pleurisy model in rats through subcutaneous administration of 50, 100, 200, and 400 mg/kg of KEPS decreased both the volume of inflammatory exudate and the number of leukocytes recruited to the pleural cavity. The present data showed that EPS production by K. oxytoca using the method described is easy to perform and results in a good yield. In addition, we show that KEPS exhibit anti-inflammatory activity when administered subcutaneously in rats |
| Disciplinas: | Química, Biología |
| Palabras clave: | Microbiología, Farmacología, Exopolisacáridos, Actividad antiinflamatoria, Pleuresia, Migración leucocitaria, Klebsiella oxytoca |
| Keyword: | Microbiology, Pharmacology, Exopolysaccharides, Anti-inflammatory activity, Pleurisy, Leukocyte migration, Klebsiella oxytoca |
| Texto completo: | Texto completo (Ver HTML) Texto completo (Ver PDF) |
