| Revista: | Brazilian Journal of Pharmaceutical Sciences |
| Base de datos: | PERIÓDICA |
| Número de sistema: | 000452094 |
| ISSN: | 1984-8250 |
| Autores: | Braga, Tatiane Vieira1 Evangelista, Fernanda Cristina Gontijo1 Santiago, Marie Gabriele1 Ferrão, Aline Lúcia Menezes1 Almeida, Tamara Dauare de1 Barbosa, Bárbara Lima da Fonseca1 Araujo, Sergio Schusterschitz da Silva2 Ribeiro, Glaciano Nogueira2 Carvalho, Maria das Graças1 Sabino, Adriano de Paula1 |
| Instituciones: | 1Universidade Federal de Minas Gerais, Faculdade de Farmacia, Belo Horizonte, Minas Gerais. Brasil 2Universidade Federal de Minas Gerais, Hospital das Clinicas, Belo Horizonte, Minas Gerais. Brasil |
| Año: | 2022 |
| Volumen: | 58 |
| País: | Brasil |
| Idioma: | Inglés |
| Tipo de documento: | Artículo |
| Enfoque: | Experimental, aplicado |
| Resumen en inglés | Chronic lymphocytic leukemia (CLL) is a blood cancer characterized by the accumulation of clonal B-lymphocytes. This study evaluated the mRNA gene expression of miR-15a, miR-16- 1, ZAP-70, and Ang-2 by qPCR, as well as the plasma levels of Bcl-2 by Elisa immunoassay, in CLL patients and healthy controls. Significant differences were observed when comparing patients and controls regarding miR-15a (p < 0.001), miR-16-1 (p < 0.001) mRNA, Ang-2 gene expression, and Bcl-2 plasma levels (p < 0.001). When stratified by risk, differences were maintained with a significantly reduced expression in high-risk patients. A positive correlation was observed between miR-15a and platelets (R2 = 0.340; p = 0.009) as well as between Bcl-2 and leukocytes (R2 = 0.310; p = 0.019). Conversely, negative correlations were observed between ZAP-70 and platelets (R2 = - 0.334; p = 0.011), between miR-15a and lymphocytes (R2 = - 0.376; p = 0.004), as well as between miR-16-and lymphocytes (R2 = - 0.515; p = 0.00004). The data suggest that a reduction in miR-15a and miR-16-1 expressions, in addition to an overexpression of Bcl-2, are associated with the reduction in apoptosis and, consequently, to a longer survival of lymphocytes, thus contributing to lymphocyte accumulation and aggravation of the disease. By contrast, Ang-2 expression was significantly higher in A than in B + C Binet groups. This context leads to the speculation that this biomarker should be investigated in more robust studies within populations with a still relevantly indolent form of the disease in an attempt to identify those patients with a greater potential for an aggravation of the disease |
| Disciplinas: | Medicina |
| Palabras clave: | Hematología, Oncología, Diagnóstico, Leucemia linfocítica crónica, Marcadores diagnósticos, Biomarcadores |
| Keyword: | Hematology, Oncology, Diagnosis, Chronic lymphocytic leukemia, Diagnostic markers, Biomarkers |
| Texto completo: | Texto completo (Ver HTML) Texto completo (Ver PDF) |
